Document Detail


Adrenergic receptors and the secretion of glucagon and insulin from the isolated, perfused canine pancreas.
MedLine Citation:
PMID:  4727451     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
During perfusion with a glucose concentration of 150 mg/100 ml, infusions of l-epinephrine, l-norepinephrine, and d-l-isoproterenol at physiological concentrations of 2 ng/ml for 9 min stimulated secretion of glucagon in a monophasic response pattern, in contrast to the biphasic response normally encountered after glucagon releasing stimuli as previously reported from our laboratory (1971. J. Clin. Invest.50: 2123). Glucagon was stimulated in spite of a glucose concentration which in itself effectively inhibits glucagon release. Release of insulin was strongly inhibited after epinephrine and norepinephrine, and strongly stimulated after isoproterenol. During perfusion with a glucose concentration of 25 mg/100 ml, secretion of glucagon was greatly accentuated by the catechols investigated. Secretion of insulin remained unchanged after epinephrine and norepinephrine, but was stimulated by isoproterenol. The catechol induced glucagon release was suppressed or abolished when the beta-blocking agent propanolol was simultaneously infused at a concentration of 1 muM, while the inhibition of insulin became further accentuated. The catechol induced glucagon release remained unchanged when alpha blockade was performed using either phentolamine (1 muM) or dibenzyline (10 mug/ml), while the inhibition of insulin was converted to a stimulation. Evidence is thus presented that both the alpha cells and the bet cells are under the influence of adrenergic substances, the stimulation of glucagon release being mediated through a beta receptor and the inhibition of insulin release being mediated through an alpha receptor.
Authors:
J Iversen
Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  The Journal of clinical investigation     Volume:  52     ISSN:  0021-9738     ISO Abbreviation:  J. Clin. Invest.     Publication Date:  1973 Sep 
Date Detail:
Created Date:  1973-11-01     Completed Date:  1973-11-01     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7802877     Medline TA:  J Clin Invest     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2102-16     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Depression, Chemical
Dogs
Epinephrine / antagonists & inhibitors,  pharmacology
Fasting
Glucagon / secretion*
Glucose / pharmacology
Insulin / secretion*
Insulin Antagonists
Isoproterenol / antagonists & inhibitors,  pharmacology
Male
Norepinephrine / antagonists & inhibitors,  pharmacology
Pancreas / drug effects,  secretion*
Perfusion
Phenoxybenzamine / pharmacology
Phentolamine / pharmacology
Propranolol / pharmacology
Receptors, Adrenergic*
Stimulation, Chemical
Chemical
Reg. No./Substance:
0/Insulin Antagonists; 0/Receptors, Adrenergic; 11061-68-0/Insulin; 50-60-2/Phentolamine; 50-99-7/Glucose; 51-41-2/Norepinephrine; 51-43-4/Epinephrine; 525-66-6/Propranolol; 59-96-1/Phenoxybenzamine; 7683-59-2/Isoproterenol; 9007-92-5/Glucagon
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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