| Adrenalectomy in mice does not prevent loss of intestinal lymphocytes after exercise. | |
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MedLine Citation:
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PMID: 15133013 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Exhaustive exercise is associated with an increase in circulating glucocorticoids (GCs), lymphocyte apoptosis, and a reduction in intestinal lymphocyte number. The present study examined the role of GCs on the numerical changes seen in intestinal lymphocytes after exercise. Female C57BL/6 mice were bilaterally adrenalectomized (ADX; n = 18) or given sham surgery (Sham; n = 18) and assigned to one of three exercise conditions: treadmill running (28 m/min, 90 min, 2 degrees slope) and killed immediately or after 24 h recovery, or not exercised and killed immediately after 90-min exposure to the treadmill environment. Lymphocytes were isolated from the intestines with CD45(+) cells collected by positive selection using magnetic bead separation columns, and lymphocyte subpopulations were analyzed by flow cytometry for CD45(+), CD3alphabeta(+), CD3gammadelta(+), CD8beta(+), CD8alpha(+), CD4(+), and NK(+) phenotypic markers. ADX mice had significantly more intestinal CD45(+) leukocytes (P < 0.05) and CD3alphabeta(+) (P < 0.05), CD3gammadelta(+) (P < 0.01), CD8alpha(+) (P < 0.001), and NK(+) (P < 0.05) intestinal lymphocytes than Sham mice. There was a significant effect of exercise condition on total intestinal CD45(+) leukocytes (P < 0.01) and CD3alphabeta(+) (P < 0.05), CD8alpha(+) (P < 0.001), and CD4(+) (P < 0.05) intestinal lymphocytes, with fewer cells at 24 h postexercise compared with the other treatment conditions. There were no surgical x exercise interaction effects on the CD3 and CD8 phenotype numbers. Plasma corticosterone was virtually nil in ADX mice regardless of exercise condition but was significantly elevated in Sham mice immediately postexercise (P < 0.001). The data indicate that ADX does not prevent the loss of lymphocytes from the intestinal mucosa 24 h after strenuous exercise and GCs are not directly causal in the leukopenia of exercise. |
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Authors:
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L Hoffman-Goetz; J Quadrilatero; J Boudreau; J Guan |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of applied physiology (Bethesda, Md. : 1985) Volume: 96 ISSN: 8750-7587 ISO Abbreviation: J. Appl. Physiol. Publication Date: 2004 Jun |
Date Detail:
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Created Date: 2004-05-10 Completed Date: 2005-01-11 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8502536 Medline TA: J Appl Physiol Country: United States |
Other Details:
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Languages: eng Pagination: 2073-81 Citation Subset: IM |
Affiliation:
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Department of Health Studies and Gerontology, Faculty of Applied Health Sciences, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adrenalectomy* Animals Antigens, Differentiation / analysis Female Intestines / cytology*, immunology, physiology Lymphocytes / cytology*, immunology Mice Mice, Inbred BALB C Models, Animal Physical Conditioning, Animal* Reference Values |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Differentiation |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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