Document Detail


Adrenalectomy alters discrete galanin mRNA levels in the hypothalamus and mesencephalon of the rat.
MedLine Citation:
PMID:  7518895     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Using solution and in situ hybridization techniques we have studied the effects of adrenalectomy with or without restitution therapy with corticosterone on galanin mRNA levels in discrete regions of the male rat brain. Galanin peptide levels were also measured using a radioimmunoassay. The solution hybridization showed a two-fold increase in galanin mRNA 7 days, but not 14 days, after adrenalectomy in the preoptic area including the hypothalamic paraventricular nucleus (PVN). No changes were observed in the mediobasal hypothalamus including the arcuate nucleus. In situ hybridization showed that the increase in galanin mRNA was localized to the PVN and that the arcuate nucleus was not affected. The changes observed could be fully counteracted by corticosterone treatment. Radioimmunoassay showed decreased galanin levels in the median eminence 14 days, but not 7 days, after adrenalectomy and increased levels in the anterior pituitary and neurointermediate lobe. The results give evidence for a regional regulation of galanin gene expression and galanin peptide synthesis by adrenocortical steroids.
Authors:
P B Hedlund; J I Koenig; K Fuxe
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neuroscience letters     Volume:  170     ISSN:  0304-3940     ISO Abbreviation:  Neurosci. Lett.     Publication Date:  1994 Mar 
Date Detail:
Created Date:  1994-08-25     Completed Date:  1994-08-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7600130     Medline TA:  Neurosci Lett     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  77-82     Citation Subset:  IM    
Affiliation:
Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Adrenalectomy*
Animals
Arcuate Nucleus / drug effects,  metabolism
Corticosterone / pharmacology
Dexamethasone / pharmacology
Galanin
Hypothalamus / drug effects,  metabolism*
In Situ Hybridization
Male
Mesencephalon / drug effects,  metabolism*
Neuropeptides / biosynthesis*
Paraventricular Hypothalamic Nucleus / drug effects,  metabolism
Peptide Biosynthesis*
RNA, Messenger / biosynthesis*
Radioimmunoassay
Raphe Nuclei / drug effects,  metabolism
Rats
Rats, Sprague-Dawley
Chemical
Reg. No./Substance:
0/Neuropeptides; 0/RNA, Messenger; 50-02-2/Dexamethasone; 50-22-6/Corticosterone; 88813-36-9/Galanin

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