| Adrenal extracellular matrix scaffolds support adrenocortical cell proliferation and function in vitro. | |
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MedLine Citation:
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PMID: 20528677 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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Transplantation of functional adrenal cortex cells could reduce morbidity and increase the quality of life of patients with adrenal insufficiency. Our aim was to determine whether adrenal extracellular matrix (ECM) scaffolds promote adrenocortical cell endocrine function and proliferation in vitro. We seeded decellularized porcine adrenal ECM with primary human fetal adrenocortical (HFA) cells. Adrenocortical function was quantified by cortisol secretion of HFA-ECM constructs after stimulation with adrenocorticotropic hormone. Proliferation was assessed by adenosine triphosphate assay. HFA-ECM construct morphology was evaluated by immunofluorescence microscopy and scanning electron microscopy. Adrenal HFA-ECM constructs coated with laminin were compared to uncoated constructs. Laminin coating did not significantly affect HFA morphology, proliferation, or function. We demonstrated HFA cell attachment to adrenal ECM scaffolds. Cortisol production and HFA cell proliferation were significantly increased in HFA-ECM constructs compared to controls (p < 0.05), and cortisol secretion rate per cell is comparable to that of human adult and fetal explants. We conclude that adrenal ECM supports endocrine function and proliferation of adrenocortical cells in vitro. Adrenal ECM scaffolds may form the basis for biocompatible tissue-engineered adrenal replacements. |
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Authors:
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Robert A Allen; Lara M Seltz; Hongbin Jiang; Rena T Kasick; Tiffany L Sellaro; Stephen F Badylak; Jennifer B Ogilvie |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-08-11 |
Journal Detail:
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Title: Tissue engineering. Part A Volume: 16 ISSN: 1937-335X ISO Abbreviation: Tissue Eng Part A Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-11-01 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101466659 Medline TA: Tissue Eng Part A Country: United States |
Other Details:
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Languages: eng Pagination: 3363-74 Citation Subset: IM |
Affiliation:
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Department of Bioengineering, University of Pittsburgh, Pittsburgh, Pennsylvania, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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