| Adoptive transfer of CD4(+)CD25(+) regulatory T cells for prevention and treatment of spontaneous abortion. | |
| | |
MedLine Citation:
|
PMID: 22261465 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
OBJECTIVES: The purpose of this study was to examine whether adoptive transfer with in vitro expanded CD4(+)CD25(+) regulatory T cells (Tregs) could prevent immune response-mediated spontaneous abortion in mice. STUDY DESIGN: Female CBA/J mice were mated with male Balb/c as the control with normal pregnancy or with DBA/2J mice as a model of spontaneous abortion. The CBA/J mice mated with DBA/2J were treated intravenously with freshly isolated or in vitro expanded Tregs on day 1 or 4 of pregnancy, respectively. The numbers of surviving and reabsorbed fetuses in the different groups of mice were counted on day 14 of pregnancy, and the concentrations of cytokines in individual sera and the supernatants of cultured Tregs were measured by ELISA. RESULTS: Adoptive transfer with freshly isolated Tregs only slightly reduced the fetal resorption rate, which was not significantly different from that of the mice without Treg treatment, regardless of treatment at early stage and implementation of pregnancy. In contrast, adoptive transfer with in vitro expanded Tregs significantly reduced the fetal resorption rates, particularly for treatment at early stage of pregnancy (P<0.05). Furthermore, adoptive transfer with in vitro expanded Tregs at early stage of pregnancy significantly increased the levels of serum IL-10, TGF-β1, and the ratios of IL-10 to IFN-γ. CONCLUSIONS: Our data clearly indicated that adoptive transfer with in vitro expanded Tregs at early stage of pregnancy protected fetuses from spontaneous abortion by re-establishing immune tolerance in mice. |
| | |
Authors:
|
Yongxiang Yin; Xiaofeng Han; Qin Shi; Yiming Zhao; Yang He |
Related Documents
:
|
21764125 - Protective effects of hydrogen-rich saline in preeclampsia rat model. 21620555 - Hydatid of morgagni: a possible underestimated cause of unexplained infertility. 21880275 - Clinical outcomes and development of children born after intracytoplasmic sperm injecti... 21895955 - Delayed blastocyst development does not influence the outcome of frozen-thawed transfer... 16828605 - Pregnancy after biventricular repair for pulmonary atresia with ventricular septal defect. 11170585 - Using an ion-trap ms sensor to differentiate and identify individual components in grap... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-1-17 |
Journal Detail:
|
Title: European journal of obstetrics, gynecology, and reproductive biology Volume: - ISSN: 1872-7654 ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
|
Created Date: 2012-1-20 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0375672 Medline TA: Eur J Obstet Gynecol Reprod Biol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2012 Elsevier Ireland Ltd. All rights reserved. |
Affiliation:
|
Maternity and Child Health Hospital, Nanjing Medical University, Wuxi, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: In situ transmission electron microscopy of light-induced photocatalytic reactions.
Next Document: Experience of the Hungarian Preconception Service between 1984 and 2010.