| Administration of recombinant human thioredoxin-1 significantly delays and prevents autoimmune diabetes in nonobese diabetic mice through modulation of autoimmunity. | |
| | |
MedLine Citation:
|
PMID: 22069264 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
BACKGROUND: Thioredoxin as a biological antioxidant plays an important role in regulating the redox system. The administration of recombinant thioredoxin has been demonstrated to be anti-inflammatory. In this study, the effect of recombinant human thioredoxin-1 (rhTrx-1) in preventing type 1 diabetes (T1D) in nonobese diabetic (NOD) mice was evaluated. METHODS: Eight-week-old NOD mice were treated with intravenous injection of rhTrx-1 (5 µg/mouse/day) for 5 weeks (5 days a week), followed by every other day for additional 5 weeks. Diabetes onset was monitored twice a week. Pancreatic histology and β-cell mass were examined by hematoxylin and eosin (H&E) and insulin immunohistochemistry staining, respectively. Adoptive transfer experiments were executed to assess autoimmune T cells modulated by rhTrx treatment. RESULTS: The intravenous administration of rhTrx-1 significantly delayed and prevented T1D in NOD mice. The histology data showed that rhTrx-1 treatment markedly reduced insulitic lesions and significantly preserved insulin-producing β cells. Adoptive transfer of spleen cells from rhTrx-1-treated mice into nonobese diabetic-severe combined immunodeficiency (NOD-SCID) mice significantly reduced the diabetes onset than transfer of those from phosphate-buffered saline-treated mice. Adoptive co-transfer experiments demonstrated that spleen cells from rhTrx-1-treated mice significantly delayed diabetes induced by the co-transferred diabetogenic spleen cells from the new-onset diabetic mice. CONCLUSIONS: Antioxidant rhTrx-1 effectively prevents T1D which may be attributed to its activity to modulate autoimmunity. Copyright © 2011 John Wiley & Sons, Ltd. |
| | |
Authors:
|
Anna V Chernatynskaya; Benjamin Looney; Hanbo Hu; Xiaoyan Zhu; Chang-Qing Xia |
Related Documents
:
|
22163064 - Can maternal microchimeric cells influence the fetal response toward self antigens? 19645834 - Evaluation of a portable meter to measure ketonemia and comparison with ketonuria for t... 2168254 - Intractable diabetic ketoacidosis due to insulin antibody--response to steroid therapy. 11208454 - Newer uses of glucose-insulin-potassium regimen. 15550674 - Fat mobilization in adipose tissue is promoted by adipose triglyceride lipase. 11106834 - Detection of the association between a deletion polymorphism in the gene encoding angio... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Diabetes/metabolism research and reviews Volume: 27 ISSN: 1520-7560 ISO Abbreviation: Diabetes Metab. Res. Rev. Publication Date: 2011 Nov |
Date Detail:
|
Created Date: 2011-11-09 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 100883450 Medline TA: Diabetes Metab Res Rev Country: England |
Other Details:
|
Languages: eng Pagination: 809-12 Citation Subset: IM |
Copyright Information:
|
Copyright © 2011 John Wiley & Sons, Ltd. |
Affiliation:
|
Department of Pathology, Immunology and Laboratory Medicine, Diabetes Center of Excellence, University of Florida, Gainesville, FL, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The combination of metallothionein and superoxide dismutase protects pancreatic ? cells from oxidati...
Next Document: Interleukin-6 treatment induces beta-cell apoptosis via STAT-3-mediated nitric oxide production.