Document Detail


Administration of quinolinic acid in the rat hippocampus induces expression of c-fos and NGFI-A.
MedLine Citation:
PMID:  1281256     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have studied the effect of intrahippocampal administration of quinolinic acid (QUIN) on the temporal expression of mRNAs encoding the immediate early genes (IEGs) c-fos and NGFI-A, by in situ hybridization histochemistry. After administration of QUIN to the left hippocampus, expression of mRNA of both IEGs was transiently stimulated. Maximal expression was found between 1 and 3 h. mRNA of both IEGs was simultaneously expressed in the ipsilateral and contralateral sides in the granule cell layer of the dentate gyrus, the pyramidal cell layer of the CA1 and CA3 fields as well as in the cortex. After pretreatment with the non-competitive NMDA antagonist MK-801 (2 mg/kg i.p. -30 min) the increased expression of both IEGs was partially prevented in the hippocampus and completely in the cortex. No inhibition was observed after treatment with the AMPA antagonist NBQX (30 mg/kg i.p. -15, -5 and +10 min). Additional delayed expression of both IEGs was observed in the ipsilateral hippocampus. This expression was related to cell damage. Twelve h after QUIN administration, c-fos and NGFI-A mRNAs were present in the dentate gyrus. After 4 days, only c-fos mRNA was observed in the dentate gyrus and CA1 field while no NGFI-A mRNA was detected. The present results show that the effect of QUIN is mediated by NMDA and not by AMPA receptors.
Authors:
L Massieu; N Rocamora; J M Palacios; G Mengod; H W Boddeke
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Brain research. Molecular brain research     Volume:  16     ISSN:  0169-328X     ISO Abbreviation:  Brain Res. Mol. Brain Res.     Publication Date:  1992 Nov 
Date Detail:
Created Date:  1993-01-11     Completed Date:  1993-01-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8908640     Medline TA:  Brain Res Mol Brain Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  88-96     Citation Subset:  IM    
Affiliation:
Preclinical Research Sandoz Pharma Ltd., Basle, Switzerland.
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MeSH Terms
Descriptor/Qualifier:
Animals
Behavior, Animal / drug effects
Dizocilpine Maleate / pharmacology*
Gene Expression Regulation / drug effects*
Genes, fos / genetics*
Hippocampus / drug effects*
Ibotenic Acid / analogs & derivatives,  antagonists & inhibitors
In Situ Hybridization
Injections, Intraperitoneal
Male
Oligonucleotide Probes
Quinolinic Acid / pharmacology*
Quinoxalines / pharmacology*
Rats
Rats, Sprague-Dawley
Receptors, N-Methyl-D-Aspartate / antagonists & inhibitors
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
Chemical
Reg. No./Substance:
0/Oligonucleotide Probes; 0/Quinoxalines; 0/Receptors, N-Methyl-D-Aspartate; 118876-58-7/2,3-dioxo-6-nitro-7-sulfamoylbenzo(f)quinoxaline; 2552-55-8/Ibotenic Acid; 77086-22-7/Dizocilpine Maleate; 77521-29-0/alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; 89-00-9/Quinolinic Acid
Comments/Corrections
Erratum In:
Brain Res Mol Brain Res 1993 May;18(3):273

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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