Document Detail


Administration of polymerized bovine hemoglobin improves survival in a rat model of traumatic shock.
MedLine Citation:
PMID:  10445236     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The ability of purified and modified hemoglobin solutions to expand blood volume and act as oxygen and carbon dioxide transporters with no apparent toxic side effects has lead to an increased interest in their utilization in shock conditions. Although hemoglobin solutions have been shown to provide beneficial effects in endotoxic and hemorrhagic shock, they have not been studied in whole body traumatic injury. We investigated the effects of a polymerized bovine hemoglobin solution (HBOC-201) in a rat model of traumatic shock. Pentobarbital-anesthetized rats subjected to Noble-Collip drum trauma developed a shock state characterized by marked hypotension, a survival time of 102 +/- 20 min, significant increases in intestinal myeloperoxidase (MPO) activity and splanchnic vascular endothelial dysfunction characterized by an impaired vasorelaxation of the superior mesenteric artery (SMA) to endothelium-dependent vasodilators. Traumatized rats were treated with HBOC-201 or an equal volume of vehicle corresponding to 5, 10 or 15% of the calculated blood volume. Treatment with HBOC-201 10 min posttrauma prolonged survival, normalized mean arterial blood pressure and attenuated endothelial dysfunction of the SMA. However, administration of HBOC-201 failed to significantly attenuate increases in intestinal MPO activity following trauma. No beneficial effects were observed with the administration the vehicle. These data indicate that HBOC-201 exerts significant beneficial effects in traumatic shock states by normalizing systemic blood pressure and attenuating vascular endothelial dysfunction. HBOC-201 may serve as a useful adjunctive agent in the early treatment of trauma.
Authors:
R Hayward; A M Lefer
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Methods and findings in experimental and clinical pharmacology     Volume:  21     ISSN:  0379-0355     ISO Abbreviation:  Methods Find Exp Clin Pharmacol     Publication Date:    1999 Jul-Aug
Date Detail:
Created Date:  1999-09-22     Completed Date:  1999-09-22     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7909595     Medline TA:  Methods Find Exp Clin Pharmacol     Country:  SPAIN    
Other Details:
Languages:  eng     Pagination:  427-33     Citation Subset:  IM    
Affiliation:
Department of Physiology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, PA, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Pressure / drug effects
Blood Substitutes / pharmacology*
Cattle
Disease Models, Animal
Hemoglobins / pharmacology*
Ileum / drug effects,  enzymology
Male
Peroxidase / metabolism
Rats
Rats, Sprague-Dawley
Shock, Traumatic / drug therapy*,  mortality*
Survival Analysis
Grant Support
ID/Acronym/Agency:
GM-45434/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Blood Substitutes; 0/HBOC 201; 0/Hemoglobins; EC 1.11.1.7/Peroxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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