Document Detail


Administration of luteinizing hormone releasing hormone agonist for synchronization of estrus and generation of pseudopregnancy for embryo transfer in rats.
MedLine Citation:
PMID:  24827564     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In the past decade, the use of genetically engineered rats has increased exponentially; therefore, the ability to perform embryo transfer (ET) in rats to rederive, reanimate, or create mutant rat lines is increasingly important. However, the successful generation of pseudopregnant female rats for ET represents a limiting factor. We here evaluated the subcutaneous administration of 40 μg luteinizing hormone releasing hormone agonist (LHRHa) for estrus synchronization during the development and implementation of a rat ET program. Our first experiment assessed endogenous estrus cycling patterns by examining vaginal cytology without administration of LHRHa in 5-wk-old peripubertal Sprague-Dawley female rats. These rats then received LHRHa at approximately 7 wk of age; 57% of the rats were synchronized in proestrus or estrus as assessed by vaginal cytology 96 h later. In a second experiment, 8-wk-old virgin, unmanipulated Sprague-Dawley female rats received LHRHa; 55% were synchronized in proestrus or estrus 96 h later. Copulatory plugs were confirmed in 28% and 82% of the rats that had been synchronized in the first and second experiments, respectively, and mated with vasectomized male rats. Embryo transfer surgery was performed, and live pups were born from both fresh and cryopreserved transgenic rat embryos. Our results indicate that subcutaneous administration of 40 μg LHRHa followed by examination of vaginal cytology 96 h later is an effective technique to generate multiple pseudopregnant recipient rats for use in an ET program.
Authors:
Tiffany M Borjeson; Jassia Pang; James G Fox; Alexis García
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of the American Association for Laboratory Animal Science : JAALAS     Volume:  53     ISSN:  1559-6109     ISO Abbreviation:  J. Am. Assoc. Lab. Anim. Sci.     Publication Date:  2014 May 
Date Detail:
Created Date:  2014-05-15     Completed Date:  2014-09-04     Revised Date:  2014-11-04    
Medline Journal Info:
Nlm Unique ID:  101269489     Medline TA:  J Am Assoc Lab Anim Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  232-7     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Embryo Transfer / methods,  veterinary*
Estrus
Female
Gonadotropin-Releasing Hormone / administration & dosage*
Male
Pseudopregnancy / chemically induced,  veterinary*
Rats*
Rats, Sprague-Dawley
Specific Pathogen-Free Organisms
Chemical
Reg. No./Substance:
33515-09-2/Gonadotropin-Releasing Hormone
Comments/Corrections

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