Document Detail

Administration of human peripheral blood-derived CD133+ cells accelerates functional recovery in a rat spinal cord injury model.
MedLine Citation:
PMID:  19148043     Owner:  NLM     Status:  MEDLINE    
STUDY DESIGN: Magnetically isolated, peripheral blood-derived CD133+ cells were used as the therapeutic agent of spinal cord injury (SCI). A rat model was used to investigate the hypothesis that the cell therapy using this clinically accessible cell fraction could be an attractive option for injured spinal cord. OBJECTIVE: Given the capacity for the peripheral blood-derived CD133+ cells in vivo to produce neurogenesis via vasculogenesis as the feasible candidate for SCI in the clinical setting, the focus of the experiment was to investigate whether the cells could contribute to histologic and functional recovery of SCI after transplantation. SUMMARY OF BACKGROUND DATA: No evidence for peripheral blood-derived CD133+ cells application to SCI and no experimental studies showed functional recovery from SCI using this cell fraction have been published. METHODS: Contusion SCI was induced by placing a 25-g rod onto the spinal cord for 90 seconds in athymic nude rats. CD133+ cells or phosphate-buffered saline was administered intravenously immediately after SCI. The animals were analyzed at specific times after transplantation by several methods to examine histologic vasculogenesis and neurogenesis and to confirm functional recovery from SCI. RESULTS: After cell transplantation, intrinsic angiogenesis and axonal regeneration were enhanced, and cavity formation was reduced in injured spinal cord, histologically, with significant functional recovery. Gene expression of vascular endothelial growth factor increased in the cell-administrated group. CONCLUSION: The administration of CD133+ cells has a therapeutic potential to a rat spinal cord injury model and could be an optional treatment for spinal cord injury in the clinical settings.
Hirofumi Sasaki; Masakazu Ishikawa; Nobuhiro Tanaka; Kazuyoshi Nakanishi; Naosuke Kamei; Takayuki Asahara; Mitsuo Ochi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Spine     Volume:  34     ISSN:  1528-1159     ISO Abbreviation:  Spine     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-02-03     Completed Date:  2009-04-21     Revised Date:  2009-07-09    
Medline Journal Info:
Nlm Unique ID:  7610646     Medline TA:  Spine (Phila Pa 1976)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  249-54     Citation Subset:  IM    
Department of Orthopaedic Surgery, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan.
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MeSH Terms
Antigens, CD / analysis*
Cell Differentiation / physiology
Cell Proliferation
Cells, Cultured
Disease Models, Animal
Endothelial Cells / immunology,  secretion,  transplantation*
Glycoproteins / analysis*
Graft Survival / physiology
Hematopoietic Stem Cell Transplantation*
Hematopoietic Stem Cells / immunology,  metabolism
Neovascularization, Physiologic / physiology
Nerve Regeneration / physiology
Neurogenesis / physiology
Peptides / analysis*
Rats, Nude
Recovery of Function / physiology
Spinal Cord Injuries / physiopathology,  therapy*
Treatment Outcome
Vascular Endothelial Growth Factor A / metabolism,  secretion
Reg. No./Substance:
0/AC133 antigen; 0/Antigens, CD; 0/Glycoproteins; 0/Peptides; 0/Vascular Endothelial Growth Factor A

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