Document Detail


Administration of factor XIII B subunit increased plasma factor XIII A subunit levels in factor XIII B subunit knock-out mice.
MedLine Citation:
PMID:  18224415     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Factor XIII (FXIII) is a proenzyme of plasma transglutaminase consisting of enzymatic A (FXIII-A) and noncatalytic B subunits (FXIII-B), and acts in hemostasis and wound healing. We freshly generated mice lacking either FXIII-A or FXIII-B to investigate the physiological functions of FXIII in vivo. Mice carrying the disrupted allele were born at the expected Mendelian ratios, and the homozygous mice were viable and fertile under specific pathogen-free conditions. Although all homozygous and heterozygous mice showed no marked difference from the wild-type animals in general appearance, homozygous mice of either FXIII-A- or FXIII-B-deficiency did have prolonged bleeding times. It was confirmed that thrombin-dependent amine incorporation and fibrin-crosslinking in plasma were undetectable in the FXIII-A-deficient mice and markedly reduced in the FXIII-B-deficient mice; however, the gene expression of each subunit was regulated independently. Recombinant human FXIII-B (rFXIII-B) was expressed in a baculovirus expression system. When rFXIII-B was injected into FXIII-B-deficient mice, FXIII-A levels, fibrin crosslinking, and amine-incorporation activities increased in their plasma, indicating that FXIII-B assisted the maintenance of FXIII-A levels in the circulation. These mouse strains will be useful in exploring the possible pathophysiological roles of each subunit in vivo.
Authors:
Masayoshi Souri; Shiori Koseki-Kuno; Naoki Takeda; Jay L Degen; Akitada Ichinose
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-12-05
Journal Detail:
Title:  International journal of hematology     Volume:  87     ISSN:  0925-5710     ISO Abbreviation:  Int. J. Hematol.     Publication Date:  2008 Jan 
Date Detail:
Created Date:  2008-01-28     Completed Date:  2008-10-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9111627     Medline TA:  Int J Hematol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  60-8     Citation Subset:  IM    
Affiliation:
Department of Molecular Patho-Biochemistry and Patho-Biology, Yamagata University School of Medicine, Yamagata, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Disease Models, Animal
Factor XIII / genetics*,  physiology,  therapeutic use
Factor XIII Deficiency / drug therapy,  genetics*,  physiopathology
Humans
Mice
Mice, Knockout
Recombinant Proteins / therapeutic use
Chemical
Reg. No./Substance:
0/Recombinant Proteins; 9013-56-3/Factor XIII; 97089-56-0/factor XIIIb

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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