Document Detail

Administration of donor-derived mesenchymal stem cells can prolong the survival of rat cardiac allograft.
MedLine Citation:
PMID:  17112896     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Mesenchymal stem cells (MSCs) are multipotent adult elements that have recently been shown to have profound immunomodulatory effects both in vitro and in vivo. Herein we have examined the impact of intravenous infusion of donor MSCs on the survival of transplanted hearts in a rat allograft model. METHODS: Recipient Fisher344 rats were transplanted with hearts from inbred Wistar rats. Wistar rat MSCs were infused via the tail vein at designated intervals. In vitro mixed lymphocyte reaction (MLR) and cell-mediated lympholysis (CML) assays were performed to assess whether MSCs downregulated T-cell responses in vivo. Real-time polymerase chain reaction (PCR) was used to analyze the Th1/Th2 balance in MSC-treated and control groups. RESULTS: The MSCs cultured in vitro exhibited multipotential for differentiation. Survival of the allografts was markedly prolonged by administration of MSCs compared with the controls, namely mean survivals of 12.4 vs 6.4 days, respectively. Real-time PCR showed a shift in the Th1/Th2 balance toward Th2. By MLR and CML assays, untreated control rats showed greater alloreactivity than did MSC-treated rats. CONCLUSION: Our results indicated that MSCs suppressed allogeneic T-cell responses both in vitro and in vivo. Intravenous administration of MSCs prolonged the survival of transplanted hearts, possibly by induction of allograft tolerance through changing the Th1/Th2 balance.
H P Zhou; D H Yi; S Q Yu; G C Sun; Q Cui; H L Zhu; J C Liu; J Z Zhang; T J Wu
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Transplantation proceedings     Volume:  38     ISSN:  0041-1345     ISO Abbreviation:  Transplant. Proc.     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2006-11-20     Completed Date:  2007-02-12     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0243532     Medline TA:  Transplant Proc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3046-51     Citation Subset:  IM    
Department of Cardiovascular Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an City, Shannxi Province, China.
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MeSH Terms
Cell Culture Techniques
DNA Primers
Graft Survival / physiology*
Heart Transplantation / immunology,  physiology*
Interferon-gamma / genetics
Interleukin-1 / genetics
Interleukin-10 / genetics
Lymphocyte Culture Test, Mixed
Mesenchymal Stem Cell Transplantation*
Mesenchymal Stem Cells / cytology
Rats, Inbred F344
Rats, Wistar
Reverse Transcriptase Polymerase Chain Reaction
Transplantation, Homologous / immunology,  physiology
Reg. No./Substance:
0/DNA Primers; 0/Interleukin-1; 130068-27-8/Interleukin-10; 82115-62-6/Interferon-gamma

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