| Administration of IL-1beta to the 4th ventricle causes anorexia that is blocked by agouti-related peptide and that coincides with activation of tyrosine-hydroxylase neurons in the nucleus of the solitary tract. | |
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MedLine Citation:
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PMID: 19028534 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Inflammation-associated cachexia is associated with multiple chronic diseases and involves activation of appetite regulating centers in the arcuate nucleus of the hypothalamus (ARH). The nucleus of the solitary tract (NTS) in the brainstem has also been implicated as an important nucleus involved in appetite regulation. We set out to determine whether the NTS may be involved in inflammation-associated anorexia by injecting IL-1 beta into the 4th ventricle and assessing food intake and NTS neuronal activation. Injection of IL-1 beta produced a decrease in food intake at 3 and 12h after injection which was ameliorated at the 12h time point by a sub-threshold dose of agouti-related peptide (AgRP). Investigation into neuron types in the NTS revealed that IL-1 beta injection was associated with an increase in c-Fos activity in NTS neurons expressing tyrosine hydroxylase (TH). Additionally, injection of IL-1 beta into the 4th ventricle did not produce c-Fos activation of neurons expressing pro-opiomelanocortin (POMC) in the ARH, cells known to be involved in producing anorexia in response to systemic inflammation. Double-label in situ hybridization revealed that TH neurons did not express IL-1 receptor I (IL1-RI) transcript, demonstrating that c-Fos activation of TH neurons in this setting was not via direct stimulation of IL-1 beta on TH neurons themselves. We conclude that IL-1 beta injection into the 4th ventricle produces anorexia and is accompanied by an increase in activation in TH neurons in the NTS. This provides evidence that the brainstem may be an important mediator of anorexia in the setting of inflammation. |
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Authors:
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Mark D DeBoer; Jarrad M Scarlett; Peter R Levasseur; Wilmon F Grant; Daniel L Marks |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2008-11-05 |
Journal Detail:
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Title: Peptides Volume: 30 ISSN: 0196-9781 ISO Abbreviation: Peptides Publication Date: 2009 Feb |
Date Detail:
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Created Date: 2009-01-21 Completed Date: 2009-05-07 Revised Date: 2013-02-12 |
Medline Journal Info:
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Nlm Unique ID: 8008690 Medline TA: Peptides Country: United States |
Other Details:
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Languages: eng Pagination: 210-8 Citation Subset: IM |
Affiliation:
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Department of Pediatrics, Oregon Health & Science University, USA. deboer@virginia.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Agouti-Related Protein
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pharmacology* Animals Anorexia / chemically induced*, metabolism Arcuate Nucleus / metabolism Brain Stem / metabolism Hypothalamus / metabolism Inflammation / metabolism Injections, Intraventricular Interleukin-1beta / administration & dosage*, pharmacology Neurons / drug effects, enzymology*, metabolism Pro-Opiomelanocortin / metabolism Rats Rats, Sprague-Dawley Solitary Nucleus / cytology, metabolism* Tyrosine 3-Monooxygenase / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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1 K08 DK062207-01/DK/NIDDK NIH HHS; F32 DK072820-01A1/DK/NIDDK NIH HHS; F32 DK072820-01A1/DK/NIDDK NIH HHS; R01 DK 70333-01/DK/NIDDK NIH HHS; R01 DK070333/DK/NIDDK NIH HHS; R01 DK070333-03/DK/NIDDK NIH HHS; R01 DK070333-04/DK/NIDDK NIH HHS; R01 DK070333-07/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Agouti-Related Protein; 0/Interleukin-1beta; 66796-54-1/Pro-Opiomelanocortin; EC 1.14.16.2/Tyrosine 3-Monooxygenase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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