| Administered CpG oligodeoxynucleotide induces mRNA expression of CXC and CC chemokines at the intestinal mucosa and PBMCs in piglets. | |
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MedLine Citation:
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PMID: 20202485 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Oligonucleotides containing CpG motifs (CpG ODN) are known to be potent stimulators of the innate immune system in vitro and in vivo. We therefore investigated if intranasal (IN)-mucosal or intramuscular (IM)-systemic administration of CpG ODN could enhance innate immunity in the intestinal mucosa and peripheral blood mononuclear cells (PBMCs) in piglets. Repeated IN or IM administration of CpG ODN significantly increased local/systemic mRNA expression of the CC chemokines macrophage inflammatory protein 1beta (MIP-1beta) and monocyte chemoattractant protein-1 (MCP-1) and CXC chemokine gamma interferon-inducible protein 10 (IP-10) and percentages of macrophages and cDCs in the intestine (jejunum, caecum and colon) and PBMCs by different kinetics. IN delivery of CpG ODN induced much stronger chemokine responses than IM delivery at intestinal mucosas, whereas IN delivery of CpG ODN induced some weaker chemokine responses than IM delivery in PBMCs. These findings suggest that IN administration of 100mug/kg-CpG ODN without antigen codelivery may represent a valuable strategy for induction of innate immunity against infection. |
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Authors:
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Qing Cheng; Chenchao Xu; Linghua Zhang; Jiaoqing Li; Ting Cao; Meirong Zhang |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2010-03-02 |
Journal Detail:
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Title: International immunopharmacology Volume: 10 ISSN: 1878-1705 ISO Abbreviation: Int. Immunopharmacol. Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-04-13 Completed Date: 2010-09-08 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100965259 Medline TA: Int Immunopharmacol Country: Netherlands |
Other Details:
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Languages: eng Pagination: 611-8 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier B.V. All rights reserved. |
Affiliation:
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College of Life Sciences, South China Agricultural University, Guangzhou 510642, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Administration, Intranasal Animals Cell Count Cell Movement / drug effects Chemokine CCL2 / biosynthesis*, genetics Chemokine CCL4 / biosynthesis*, genetics CpG Islands / genetics Dendritic Cells / pathology Immunity, Innate Injections, Intramuscular Intestinal Mucosa / drug effects*, immunology, metabolism, pathology Leukocytes, Mononuclear / drug effects, immunology, metabolism, pathology Macrophages / pathology Oligodeoxyribonucleotides / administration & dosage*, genetics Pyrimidinones / metabolism RNA, Messenger / analysis* Swine Thiazoles / metabolism Vaccination |
| Chemical | |
Reg. No./Substance:
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0/Chemokine CCL2; 0/Chemokine CCL4; 0/Oligodeoxyribonucleotides; 0/Pyrimidinones; 0/RNA, Messenger; 0/Thiazoles; 89367-92-0/5(4'-hydroxybenzylidenoimino)-3-methylisothiazolo(5,4-d)pyrimidine-(7H)-4,6-dione |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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