Document Detail


Adjuvant induced glucose uptake by activated T cells is not correlated with increased survival.
MedLine Citation:
PMID:  18290315     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Authors contributed equally to this manuscript Natural adjuvants, such as bacterial lipopolysaccharide (LPS), activate antigen presenting cells via Toll-like receptors and, indirectly, increase the survival of antigen-activated T cells. The molecular mechanisms leading to increased survival remain poorly defined. Because T cell clonal expansion leads to high energy demands, we hypothesized that increased glucose uptake and/or utilization in adjuvant-activated T cells could be important molecular event(s) that would lead to adjuvant-associated T cell survival advantage. Using a fluorescent analog of 2-deoxyglucose, 2-NBDG, we measured glucose accumulation and rate of uptake in T cells from mice treated with antigen in the absence or presence of LPS. Although adjuvant activated T cells increased the accumulation of 2-NBDG, the rate of uptake was unchanged compared to cells activated with only antigen. Furthermore, glucose transport inhibitors, cytochalasin B or phloretin, decreased the accumulation of glucose in adjuvant-treated T cells, but this decrease did not impair adjuvant-associated survival advantages. Together, these data indicate that increased glucose uptake through glucose transporters is not required for increased survival of activated T cells.
Authors:
Sadhak Sengupta; Rebecca J Vitale; Paula M Chilton; Thomas C Mitchell
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Advances in experimental medicine and biology     Volume:  614     ISSN:  0065-2598     ISO Abbreviation:  Adv. Exp. Med. Biol.     Publication Date:  2008  
Date Detail:
Created Date:  2008-02-22     Completed Date:  2008-04-04     Revised Date:  2010-12-17    
Medline Journal Info:
Nlm Unique ID:  0121103     Medline TA:  Adv Exp Med Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  65-72     Citation Subset:  IM    
Affiliation:
Institute for Cellular Therapeutics, University of Louisville School of Medicine, 570, S. Preston Street, Louisville, KY 40202, USA.
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MeSH Terms
Descriptor/Qualifier:
4-Chloro-7-nitrobenzofurazan / analogs & derivatives,  metabolism
Adjuvants, Immunologic / pharmacology*
Animals
Biological Transport / drug effects
CD4-Positive T-Lymphocytes / metabolism
Cell Survival / drug effects
Cells, Cultured
Cytochalasin B / pharmacology
Dose-Response Relationship, Drug
Enterotoxins / pharmacology
Glucosamine / analogs & derivatives,  metabolism
Glucose / analysis,  antagonists & inhibitors,  metabolism*
Lipopolysaccharides / pharmacology
Luminescent Measurements
Lymphocyte Activation / drug effects*
Mice
Mice, Inbred Strains
Phloretin / pharmacology
T-Lymphocytes / cytology*,  drug effects*
Time Factors
Grant Support
ID/Acronym/Agency:
R01 AI071047-04/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Adjuvants, Immunologic; 0/Enterotoxins; 0/Lipopolysaccharides; 10199-89-0/4-Chloro-7-nitrobenzofurazan; 108708-22-1/6-deoxy-N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)aminoglucose; 14930-96-2/Cytochalasin B; 3416-24-8/Glucosamine; 37337-57-8/enterotoxin A, Staphylococcal; 50-99-7/Glucose; 60-82-2/Phloretin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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