Document Detail


Adjuvant activity of 6-O-acyl-muramyldipeptides to enhance primary cellular and humoral immune responses in guinea pigs: adaptability to various vehicles and pyrogenicity.
MedLine Citation:
PMID:  3744548     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Thirteen 6-O-acyl-N-acetylmuramyl-L-alanyl-D-isoglutamines (6-O-acyl-MDPs), including four inactive D-isoasparagine and L-isoglutamine analogs, were tested for their pyrogenicity and immunopotentiating activity to stimulate primary humoral and cellular immune responses in guinea pigs to a model protein antigen, ovalbumin, when administered in various vehicles. Among them, derivatives whose muramic acid residue was substituted by alpha-branched (and beta-hydroxylated) higher fatty acids at the carbon-6 position, especially 6-O-(2-tetradecylhexadecanoyl)-MDP (B3O-MDP) and, to a lesser extent, 6-O-(3-hydroxy-2-docosylhexacosanoyl)-MDP (BH48-MDP) and its L-serine analog [BH48-MDP(L-Ser)], were found to exert strong adjuvant activity in both the induction of delayed-type hypersensitivity and the stimulation of circulating precipitating antibody levels when combined with nonirritating vehicles (liposomes, squalene-in-water emulsion, and phosphate-buffered saline). These vehicles did not efficiently support the adjuvant activity of MDP, the parent molecule of the above lipophilic derivatives. Pyrogenicity tests showed that introduction of alpha-branched higher fatty acid groups but not of straight, long-chain fatty acids at the 6-position of the muramic acid residue resulted in marked decrease of the pyrogenicity inherent to MDP via intravenous administration.
Authors:
M Tsujimoto; S Kotani; F Kinoshita; S Kanoh; T Shiba; S Kusumoto
Related Documents :
6197148 - Guinea-pig alpha 1-fetoprotein: purification, characterization, developmental and hormo...
1971818 - Role of leukotrienes during oleic acid-induced lung injury in pigs.
1685528 - Changes in k(+)-induced contractions in ileal longitudinal smooth muscle of guinea-pig,...
2547638 - Benzofuran analogues of baclofen: a new class of central and peripheral gabab-receptor ...
7813398 - On the use of dimensionless parameters in acid-base theory. vii. the ph of solutions of...
18071638 - Purification and characterization of aprotinin from porcine lungs.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Infection and immunity     Volume:  53     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  1986 Sep 
Date Detail:
Created Date:  1986-10-15     Completed Date:  1986-10-15     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  511-6     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Acetylmuramyl-Alanyl-Isoglutamine / analogs & derivatives*,  pharmacology
Adjuvants, Immunologic / pharmacology*,  toxicity
Animals
Antibody Formation / drug effects*
Emulsions
Female
Fever / chemically induced*
Guinea Pigs
Immunity, Cellular / drug effects*
Liposomes / administration & dosage
Rabbits
Structure-Activity Relationship
Vehicles
Chemical
Reg. No./Substance:
0/Adjuvants, Immunologic; 0/Emulsions; 0/Liposomes; 0/Vehicles; 53678-77-6/Acetylmuramyl-Alanyl-Isoglutamine
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Antibody-independent activation of the classical pathway of human serum complement by lipid A is res...
Next Document:  Adjuvant activity of 6-O-acyl-muramyldipeptides to enhance primary cellular and humoral immune respo...