Document Detail

Adjusting interphase FISH results in epithelial tissue sections to whole cell complement.
MedLine Citation:
PMID:  11332084     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To derive an equation to compensate for the discrepancies between whole cell preparations and tissue sections for more accurate enumeration of fluorescence in situ hybridization (FISH) signals per cell.
STUDY DESIGN: Mean centromere signal counts in touch preparations and corresponding 4-6-micron sections of paraffin-embedded tissue were calculated. Mean widest nuclear diameters were also determined from the tissue sections. The observed data were analyzed to define the volumetric relationships between tissue sections and whole cell preparations.
RESULTS: Analysis of results from six lung specimens yielded an equation that approximates whole versus sectioned nuclear volume and permits accurate quantification of mean FISH signal count in histologic sections, as follows: [formula: see text].
M Berezuk; J West; M Varella-Garcia; W A Franklin
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Analytical and quantitative cytology and histology / the International Academy of Cytology [and] American Society of Cytology     Volume:  23     ISSN:  0884-6812     ISO Abbreviation:  Anal. Quant. Cytol. Histol.     Publication Date:  2001 Apr 
Date Detail:
Created Date:  2001-05-02     Completed Date:  2001-12-04     Revised Date:  2014-11-05    
Medline Journal Info:
Nlm Unique ID:  8506819     Medline TA:  Anal Quant Cytol Histol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  93-100     Citation Subset:  IM    
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MeSH Terms
Carcinoma / pathology*
Cell Nucleus / ultrastructure*
Cell Size*
DNA, Neoplasm / analysis
Epithelial Cells / ultrastructure*
In Situ Hybridization, Fluorescence / methods*
Lung / cytology*
Lung Neoplasms / pathology*
Grant Support
Reg. No./Substance:
0/DNA, Neoplasm

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