Document Detail


Adjacent basic amino acid residues recognized by the COP I complex and ubiquitination govern endoplasmic reticulum to cell surface trafficking of the nicotinic acetylcholine receptor alpha-Subunit.
MedLine Citation:
PMID:  11279119     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The nicotinic acetylcholine receptor in muscle is a ligand-gated ion channel with an ordered subunit arrangement of alpha-gamma-alpha-delta-beta. The subunits are sequestered in the endoplasmic reticulum (ER) and assembled into the pentameric arrangement prior to their exit to the cell surface. Mutating the Arg(313)-Lys(314) sequence in the large cytoplasmic loop of the alpha-subunit to K314Q promotes the trafficking of the mutant unassembled alpha-subunit from the ER to the Golgi in transfected HEK cells, identifying an important determinant that modulates the ER to Golgi trafficking of the subunit. The association of the K314Q alpha-subunit with gamma-COP, a component of COP I coats implicated in Golgi to ER anterograde transport, is diminished to a level comparable to that observed for wild-type alpha-subunits when co-expressed with the beta-, delta-, and gamma-subunits. This suggests that the Arg(313)-Lys(314) sequence is masked when the subunits assemble, thereby enabling ER to Golgi trafficking of the alpha-subunit. Although unassembled K314Q alpha-subunits accumulate in the Golgi, they are not detected at the cell surface, suggesting that a second post-Golgi level of capture exists. Expressing the K314Q alpha-subunit in the absence of the other subunits in ubiquitinating deficient cells (ts20) results in detecting this subunit at the cell surface, indicating that ubiquitination functions as a post-Golgi modulator of trafficking. Taken together, our findings support the hypothesis that subunit assembly sterically occludes the trafficking signals and ubiquitination at specific sites. Following the masking of these signals, the assembled ion channel expresses at the cell surface.
Authors:
S H Keller; J Lindstrom; M Ellisman; P Taylor
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.     Date:  2001-02-15
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  276     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2001 May 
Date Detail:
Created Date:  2001-05-23     Completed Date:  2001-07-05     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  18384-91     Citation Subset:  IM    
Affiliation:
Department of Pharmacology, University of California, San Diego, La Jolla, Califronia 92093, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Transport
Cell Line
Coat Protein Complex I / metabolism*
Endoplasmic Reticulum / metabolism*
Golgi Apparatus / metabolism
Mice
Receptors, Nicotinic / metabolism*
Signal Transduction
Ubiquitins / metabolism
Grant Support
ID/Acronym/Agency:
GM18360/GM/NIGMS NIH HHS; NS11323/NS/NINDS NIH HHS; RR P41-04050/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Coat Protein Complex I; 0/Receptors, Nicotinic; 0/Ubiquitins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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