| Adipose tissue transcriptome changes during obesity development in female dogs. | |
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MedLine Citation:
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PMID: 21224421 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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During the development of obesity, adipose undergoes major expansion and remodeling, but the biological processes involved in this transition are not well understood. The objective of this study was to analyze global gene expression profiles of adipose tissue in dogs, fed a high-fat diet, during the transition from a lean to obese phenotype. Nine female beagles (4.09 ± 0.64 y; 8.48 ± 0.35 kg) were randomized to ad libitum feeding or bodyweight maintenance. Subcutaneous adipose tissue biopsy, blood, and dual x-ray absorptiometry measurements were collected at 0, 4, 8, 12, and 24 wk of feeding. Serum was analyzed for glucose, insulin, fructosamine, triglycerides, free fatty acids, adiponectin and leptin. Formalin fixed adipose tissue was used for determination of adipocyte size. Adipose RNA samples were hybridized to Affymetrix Canine 2.0 microarrays. Statistical analysis using repeated measures ANOVA, showed ad libitum feeding increased (P<0.05) bodyweight (0 wk: 8.36 ± 0.34; 24 kg wk: 14.64 ± 0.34 kg), body fat mass (0 wk: 1.36 ± 0.24 kg; 24 wk: 6.52 ± 0.24 kg), adipocyte size (0 wk: 114.66 ± 17.38 μm(2); 24 wk: 320.97 ± 0.18.17 μm(2)), and leptin (0 wk: 0.8 ± 1.0 ng/ml; 24 wk: 12.9 ± 1.0 ng/ml). Microarrays displayed 1665 differentially expressed genes in adipose tissue as weight increased. Alterations were seen in adipose tissue homeostatic processes including metabolism, oxidative stress, mitochondrial homeostasis, and extracellular matrix. Adipose transcriptome changes highlight the dynamic and adaptive response to ad libitum feeding and obesity development. |
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Authors:
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Ryan W Grant; Brittany M Vester Boler; Tonya K Ridge; Thomas K Graves; Kelly S Swanson |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-1-11 |
Journal Detail:
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Title: Physiological genomics Volume: - ISSN: 1531-2267 ISO Abbreviation: - Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2011-1-12 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9815683 Medline TA: Physiol Genomics Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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University of Illinois. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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