| Adipose-derived stem cells on hyaluronic Acid-derived scaffold: a new horizon in bioengineered cornea. | |
| | |
MedLine Citation:
|
PMID: 22332213 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
OBJECTIVE: To evaluate the ability of human adipose-derived stem cells (h-ASCs) to survive and differentiate in corneal stroma. METHODS: Our experiment consisted of 2 phases. First, we cultured h-ASCs in different types of hyaluronic acid (HA)-derived synthetic extracellular matrixes (sECMs) to determine the capability of proliferation and survival of the cells in hydrogels. Second, h-ASCs were grown in plastic flasks, labeled with an intracytoplasmic membrane fluorescent molecule, transferred onto different types of sECMs or the native HA product, and then inserted into the corneal stroma of the rabbits. After 10 weeks, we assessed the viability of the stem cells and the expression of cornea-specific proteins. RESULTS: The in vitro study showed that the HyStem-HP hydrogel had the highest yield of cells (1.1 × 10(6)/mL) compared with other types of HA-derived sECMs culture media, and the cells grown in the HyStem-HP hydrogel appeared more elongated and fibroblastlike. The in vivo study demonstrated that the labeled h-ASCs could be identified in the stroma with any type of sECM. The HA-derived sECMs, particularly the HyStem-HP hydrogel, showed better survival and cell morphologic features compared with pure HA. Immunostaining of keratocan, aldehyde dehydrogenase, and type I collagen revealed that the stem cells had expressed human cornea-specific proteins. CONCLUSION: Human adipose-derived stem cells can be successfully grown on HA-derived sECMs in vivo and can express human cornea-specific proteins. Clinical Relevance Human ASCs on an HA-derived scaffold may be used as a source of keratocytes to regenerate extracellular matrix-like material in situations where the cornea stroma has been compromised. |
| | |
Authors:
|
Ladan Espandar; Bruce Bunnell; Guo Yong Wang; Paula Gregory; Christine McBride; Majid Moshirfar |
Related Documents
:
|
22392973 - Scanning probe-enabled nanocombinatorics define the relationship between fibronectin fe... 22291913 - A flexible model of hiv-1 latency permitting evaluation of many primary cd4 t-cell rese... 1847983 - Establishment of a lymphoblastoid cell line, sd-1, expressing the p190 bcr-abl chimaeri... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Archives of ophthalmology Volume: 130 ISSN: 1538-3601 ISO Abbreviation: Arch. Ophthalmol. Publication Date: 2012 Feb |
Date Detail:
|
Created Date: 2012-02-14 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7706534 Medline TA: Arch Ophthalmol Country: United States |
Other Details:
|
Languages: eng Pagination: 202-8 Citation Subset: AIM; IM |
Affiliation:
|
Department of Ophthalmology, Tulane University, SL-69, 1430 Tulane Ave, New Orleans, LA 70112. lespanda@tulane.edu. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Superior rectus transposition and medial rectus recession for Duane syndrome and sixth nerve palsy.
Next Document: Association of IGF1 Gene Haplotypes With High Myopia in Chinese Adults.