| Adipose aspirates as a source for human processed lipoaspirate cells after optimal cryopreservation. | |
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MedLine Citation:
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PMID: 16651957 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: The purpose of this study was to test the authors' hypothesis that previously cryopreserved adipose aspirates collected from conventional liposuction could still be a reliable source of human processed lipoaspirate cells. METHODS: Adipose aspirates were collected from 12 adult female patients after conventional liposuction of the abdomen and were then preserved by an optimal cryopreservation method with added cryoprotective agents (0.5 M dimethyl sulfoxide and 0.2 M trehalose). Cryopreservation of the adipose tissues was subsequently conducted with controlled slow cooling and then stored in liquid nitrogen (-196 degrees C). One gram of fresh or cryopreserved (after fast rewarming) adipose aspirates was processed in vitro and the resulting cell pellet, consisting of processed lipoaspirate cells, was cultured separately. The length of time until processed lipoaspirate cells became adherent to the culture plate was recorded and the number of processed lipoaspirate cells after a 2-week culture was counted. RESULTS: Flat, spindle-shape processed lipoaspirate cells from the cryopreserved group became adherent to the plate within 48 to 72 hours after initial culture compared with the fresh group, where the cells became adherent by 24 hours. After a 2-week culture, the cryopreserved aspirates yielded an average of 3.7 +/- 1.4 x 10(5) processed lipoaspirate cells per milliliter, equal to 90 percent of the yielded number of cells obtained from the fresh aspirates (4.1 +/- 1.4 x 10(5) cells/ml). CONCLUSIONS: The authors' results indicate that although there is a latency of cell growth after an optimal cryopreservation, cryopreserved adipose aspirates can yield a significant number of processed lipoaspirate cells compared with fresh aspirates and may be a reliable source of human processed lipoaspirate cells because they can still be processed later after long-term preservation. |
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Authors:
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Lee L Q Pu; Xiangdong Cui; Betsy F Fink; Dayong Gao; Henry C Vasconez |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Plastic and reconstructive surgery Volume: 117 ISSN: 1529-4242 ISO Abbreviation: Plast. Reconstr. Surg. Publication Date: 2006 May |
Date Detail:
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Created Date: 2006-05-02 Completed Date: 2006-05-23 Revised Date: 2011-02-16 |
Medline Journal Info:
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Nlm Unique ID: 1306050 Medline TA: Plast Reconstr Surg Country: United States |
Other Details:
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Languages: eng Pagination: 1845-50 Citation Subset: AIM; IM |
Affiliation:
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Division of Plastic Surgery, Department of Mechanical Engineering, University of Kentucky, Lexington, KY, USA. lpu@uky.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adipocytes
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cytology*,
drug effects Adult Cell Division Cell Separation / methods Cell Shape Cell Survival Cells, Cultured / cytology Cryopreservation / methods* Cryoprotective Agents / pharmacology Dimethyl Sulfoxide / pharmacology Female Humans Lipectomy* Middle Aged Subcutaneous Fat, Abdominal / cytology* Suction Tissue and Organ Harvesting / methods* Trehalose / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Cryoprotective Agents; 67-68-5/Dimethyl Sulfoxide; 99-20-7/Trehalose |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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