Document Detail

Adipose Stromal Vascular Fraction Cells Isolated using an Automated Point of Care System Improve the Patency of ePTFE Vascular Grafts.
MedLine Citation:
PMID:  23350681     Owner:  NLM     Status:  Publisher    
We evaluated the use of an automated, point-of-care instrument to derive canine adipose stromal vascular fraction cells, and the subsequent deposition of these cells onto the luminal surface of an expanded polytetrafluoroethylene (ePTFE) vascular graft for use as a bypass graft. The hypothesis evaluated was that an instrument requiring minimal user interface will provide a therapeutic dose of cells to improve the patency of synthetic vascular grafts in an autologous animal model of graft patency. The SVF cells were isolated using an automated adipose tissue processing and cell isolation system and cells sodded onto the surface of an ePTFE vascular graft. Control grafts, used off-the-shelf without cell treatment were used as a control to assess patency effects. Each animal received a control, untreated graft implanted in one carotid artery and the cell-treated graft implanted in the carotid artery on the contra-lateral side. The grafts were implanted for 6 months utilizing 12 animals. Results indicate a fully automated adipose tissue processing system will consistently produce functional autologous cells for immediate use in the operating room. Cell-sodded polymeric grafts exhibited improved patency compared to control grafts following six month implantation in the canine carotid artery model.
Stuart K Williams; Paul Kosnik; Leigh B Kleinert; Erik Vossman; Kevin Lye
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-25
Journal Detail:
Title:  Tissue engineering. Part A     Volume:  -     ISSN:  1937-335X     ISO Abbreviation:  Tissue Eng Part A     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101466659     Medline TA:  Tissue Eng Part A     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Cardiovascular Innovation Institute, 302 E Muhammad Ali Blvd, Louisville, Kentucky, United States, 40202, 5028521379, 5028521391;
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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