| Adiponectin receptor expression is elevated in colorectal carcinomas but not in gastrointestinal stromal tumors. | |
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MedLine Citation:
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PMID: 18310295 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Circulating adiponectin is inversely associated with colorectal carcinoma. However, adiponectin receptor expression has not been examined in normal gastrointestinal tissue, colorectal malignancies, or gastrointestinal stromal tumors (GISTs). We collected 40 colorectal carcinomas and 12 non-tumor colorectal tissue specimens from patients with colorectal cancer, as well as 45 tumor and 13 non-tumor specimens from patients with GIST. Expression and localization of adiponectin receptors (AdipoR1 and AdipoR2) were assessed using immunohistochemistry. We also confirmed expression of adiponectin receptors using rtPCR in matched normal and colorectal cancer specimens obtained from five patients. Finally, we detected adiponectin receptors and assessed adiponectin signaling in three colon cancer cell lines. Adiponectin receptor expression, assessed by either rtPCR or immunohistochemistry, was present in normal tissue and was significantly lower than in colorectal carcinomas. Among carcinomas, 95% displayed positive or strongly positive expression of AdipoR1 and 88% of AdipoR2, versus 8% and 0%, respectively, for non-tumor specimens (P<0.0001). AdipoR1 expression assessed by rtPCR was 1.6-fold higher in tumor than in non-tumor tissue (P<0.05). In addition, we found that adiponectin at physiological concentrations can activate in vitro intracellular signaling pathways in three colon cancer cell lines, expressing both adiponectin receptors 1 and 2. No significant differences in expression of adiponectin receptors in tumor versus non-tumor GI specimens were detected among patients with GIST. Colon cancer cell lines express adiponectin receptors, through which adiponectin activates in vitro intracellular signaling pathways. Adiponectin receptors are also detected in normal GI tissue and their expression is elevated in colorectal carcinomas, but not in GIST. |
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Authors:
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Catherine J Williams; Nicholas Mitsiades; Elias Sozopoulos; Alex Hsi; Alicja Wolk; Artemissia-Phoebe Nifli; Sofia Tseleni-Balafouta; Christos S Mantzoros |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Endocrine-related cancer Volume: 15 ISSN: 1351-0088 ISO Abbreviation: Endocr. Relat. Cancer Publication Date: 2008 Mar |
Date Detail:
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Created Date: 2008-03-03 Completed Date: 2008-05-08 Revised Date: 2008-05-15 |
Medline Journal Info:
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Nlm Unique ID: 9436481 Medline TA: Endocr Relat Cancer Country: England |
Other Details:
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Languages: eng Pagination: 289-99 Citation Subset: IM |
Affiliation:
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Division of Endocrinology, Diabetes and Metabolism, Department of Internal Medicine, Harvard Medical School, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Blotting, Western Cell Proliferation Colorectal Neoplasms / genetics*, metabolism Female Gastrointestinal Stromal Tumors / genetics*, metabolism Gastrointestinal Tract / metabolism, pathology Humans Immunoenzyme Techniques Male Middle Aged RNA, Messenger / genetics, metabolism Receptors, Adiponectin / genetics*, metabolism Reverse Transcriptase Polymerase Chain Reaction |
| Chemical | |
Reg. No./Substance:
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0/ADIPOR1 protein, human; 0/ADIPOR2 protein, human; 0/RNA, Messenger; 0/Receptors, Adiponectin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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