| Adiponectin inhibits spontaneous and catecholamine-induced lipolysis in human adipocytes of non-obese subjects through AMPK-dependent mechanisms. | |
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MedLine Citation:
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PMID: 20945960 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Adiponectin is an adipokine increasing glucose and fatty acid metabolism and improving insulin sensitivity. The aim of this study was to investigate the role of adiponectin in the regulation of adipocyte lipolysis. Human adipocytes isolated from biopsies obtained during surgical operations from 16 non-obese and 17 obese subjects were incubated with 1) human adiponectin (20 microg/ml) or 2) 0.5 mM AICAR - activator of AMPK (adenosine monophosphate activated protein kinase). Following these incubations, isoprenaline was added (10(-6) M) to investigate the influence of adiponectin and AICAR on catecholamine-induced lipolysis. Glycerol concentration was measured as lipolysis marker. We observed that adiponectin suppressed spontaneous lipolysis by 21 % and isoprenaline-induced lipolysis by 14 % in non-obese subjects. These effects were not detectable in obese individuals, but statistically significant differences in the effect of adiponectin between obese and non-obese were not revealed by two way ANOVA test. The inhibitory effect of AICAR and adiponectin on lipolysis was reversed by Compound C. Our results suggest, that adiponectin in physiological concentrations inhibits spontaneous as well as catecholamine-induced lipolysis. This effect might be lower in obese individuals and this regulation seems to involve AMPK. |
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Authors:
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Z Wedellová; J Dietrich; M Siklová-Vítková; K Kološtová; M Kováčiková; M Dušková; J Brož; T Vedral; V Stich; J Polák |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-10-15 |
Journal Detail:
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Title: Physiological research / Academia Scientiarum Bohemoslovaca Volume: 60 ISSN: 1802-9973 ISO Abbreviation: Physiol Res Publication Date: 2011 |
Date Detail:
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Created Date: 2011-04-07 Completed Date: 2011-08-09 Revised Date: 2012-04-09 |
Medline Journal Info:
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Nlm Unique ID: 9112413 Medline TA: Physiol Res Country: Czech Republic |
Other Details:
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Languages: eng Pagination: 139-48 Citation Subset: IM |
Affiliation:
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Third Faculty of Medicine of Charles University, Sport Medicine Department, Prague, Czech Republic. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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AMP-Activated Protein Kinases
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metabolism* Adipocytes / drug effects, enzymology* Adiponectin / pharmacology Humans Isoproterenol / pharmacology* Lipolysis / drug effects Male Middle Aged Obesity / metabolism |
| Chemical | |
Reg. No./Substance:
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0/ADIPOQ protein, human; 0/Adiponectin; 7683-59-2/Isoproterenol; EC 2.7.11.1/AMP-Activated Protein Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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