| Adiponectin, a fat cell product, influences the earliest lymphocyte precursors in bone marrow cultures by activation of the cyclooxygenase-prostaglandin pathway in stromal cells. | |
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MedLine Citation:
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PMID: 14607907 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Adiponectin, an adipocyte-derived hormone, is attracting considerable interest as a potential drug for diabetes and obesity. Originally cloned from human s.c. fat, the protein is also found in bone marrow fat cells and has an inhibitory effect on adipocyte differentiation. The aim of the present study is to explore possible influences on lymphohematopoiesis. Recombinant adiponectin strongly inhibited B lymphopoiesis in long-term bone marrow cultures, but only when stromal cells were present and only when cultures were initiated with the earliest category of lymphocyte precursors. Cyclooxygenase inhibitors abrogated the response of early lymphoid progenitors to adiponectin in stromal cell-containing cultures. Furthermore, PGE(2), a major product of cyclooxygenase-2 activity, had a direct inhibitory influence on purified hematopoietic cells, suggesting a possible mechanism of adiponectin action in culture. In contrast to lymphopoiesis, myelopoiesis was slightly enhanced in adiponectin-treated bone marrow cultures, and even when cultures were initiated with single lymphomyeloid progenitors. Finally, human B lymphopoiesis was also sensitive to adiponectin in stromal cell cocultures. These results suggest that adiponectin can negatively and selectively influence lymphopoiesis through induction of PG synthesis. They also indicate ways that adipocytes in bone marrow can contribute to regulation of blood cell formation. |
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Authors:
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Takafumi Yokota; C S Reddy Meka; Taku Kouro; Kay L Medina; Hideya Igarashi; Masahiko Takahashi; Kenji Oritani; Tohru Funahashi; Yoshiaki Tomiyama; Yuji Matsuzawa; Paul W Kincade |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of immunology (Baltimore, Md. : 1950) Volume: 171 ISSN: 0022-1767 ISO Abbreviation: J. Immunol. Publication Date: 2003 Nov |
Date Detail:
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Created Date: 2003-11-10 Completed Date: 2004-02-18 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 2985117R Medline TA: J Immunol Country: United States |
Other Details:
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Languages: eng Pagination: 5091-9 Citation Subset: AIM; IM |
Affiliation:
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Immunobiology and Cancer Program, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adipocytes
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physiology* Adiponectin Animals B-Lymphocyte Subsets / cytology, physiology Bone Marrow Cells / enzymology, metabolism* Cell Line Cell Lineage / immunology, physiology Cells, Cultured Coculture Techniques Enzyme Activation / immunology, physiology Growth Inhibitors / pharmacology, physiology Hematopoietic Stem Cells / cytology, enzymology, metabolism* Humans Intercellular Signaling Peptides and Proteins* Lymphocyte Subsets / cytology, enzymology, metabolism* Lymphopoiesis / immunology, physiology Mice Mice, Inbred BALB C Myeloid Cells / cytology, physiology Myelopoiesis / immunology, physiology Prostaglandin-Endoperoxide Synthases / metabolism*, physiology Prostaglandins / physiology Protein Biosynthesis Proteins / pharmacology, physiology* Signal Transduction / immunology, physiology* Stromal Cells / enzymology, metabolism |
| Grant Support | |
ID/Acronym/Agency:
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AI 20069/AI/NIAID NIH HHS; AI 45864/AI/NIAID NIH HHS; AI33085/AI/NIAID NIH HHS; P20 RR 15577/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Adiponectin; 0/Growth Inhibitors; 0/Intercellular Signaling Peptides and Proteins; 0/Prostaglandins; 0/Proteins; EC 1.14.99.1/Prostaglandin-Endoperoxide Synthases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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