Document Detail

Adipogenic differentiation of human adipose-derived stem cells on 3D silk scaffolds.
MedLine Citation:
PMID:  21082412     Owner:  NLM     Status:  MEDLINE    
Current treatment modalities for soft tissue defects due to various pathologies and trauma include autologous grafting and the use of commercially available fillers. However, these treatment methods are associated with a number of limitations, such as donor site morbidity and volume loss over time. As such, improved therapeutic options are needed. Tissue engineering techniques offer novel solutions to these problems through development of bioactive tissue constructs that can regenerate adipose tissue with an appropriate structure and function. The recent advances in the derivation and characterization of hASCs have led to numerous studies of soft tissue reconstruction. In this chapter, we discuss methods in which our laboratory has used hASCs and silk scaffolds for adipose tissue engineering. The use of naturally occurring and clinically acceptable materials such as silk protein for tissue-engineering applications poses advantages with respect to biocompatibility and mechanical and biological properties.
Jennifer H Choi; Evangelia Bellas; Gordana Vunjak-Novakovic; David L Kaplan
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  702     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2011  
Date Detail:
Created Date:  2010-11-18     Completed Date:  2011-02-23     Revised Date:  2014-08-21    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  319-30     Citation Subset:  IM    
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MeSH Terms
Adipocytes / cytology,  drug effects,  metabolism
Adipogenesis / drug effects*
Adipose Tissue / cytology*
Biocompatible Materials / pharmacology
Cell Proliferation / drug effects
Coculture Techniques
Endothelial Cells / cytology,  drug effects,  metabolism
Leptin / secretion
Lipogenesis / drug effects
Lipolysis / drug effects
Microscopy, Confocal
Silk / pharmacology*
Stem Cells / cytology*,  drug effects*,  metabolism
Tissue Engineering / methods*
Tissue Scaffolds / chemistry*
Grant Support
Reg. No./Substance:
0/Biocompatible Materials; 0/Leptin; 0/Silk; 0/Solvents

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