| Adipocyte differentiation of human marrow mesenchymal stem cells reduces the supporting capacity for hematopoietic progenitors but not for severe combined immunodeficiency repopulating cells. | |
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MedLine Citation:
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PMID: 17273785 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Bone marrow stromal cells provide a microenvironment for hematopoiesis. Adipocytes are the major stromal cell phenotype in bone marrow, but their function in hematopoiesis is poorly understood. In this study, we compared the hematopoietic-supporting capacity of adipocytes and their progenitor, mesenchymal stem cells (MSCs), by culturing human cord blood (CB) CD34+CD38- hematopoietic progenitor cells (HPCs) on a layer of adipocytes or MSCs. CB CD34+CD38- cells cultured on MSCs generated higher proportions of CD34+CD38- HPCs and colony-forming cells than those cultures on a layer of adipocytes, indicating an inferior hematopoietic support by adipocytes. However, CB CD34+CD38- HPCs cultured on MSCs and adipocytes were equally capable of reconstituting human hematopoiesis in non-obese diabetic/severe combined immunodeficient disease (NOD/SCID) mice. These findings show that differentiation of MSCs into adipocytes is accompanied by the loss of capacity to support mature HPCs, but not transplantable SCID-repopulating cells. |
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Authors:
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Nobuhiko Ookura; Yoshihiro Fujimori; Keisuke Nishioka; Shunro Kai; Hiroshi Hara; Hiroyasu Ogawa |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: International journal of molecular medicine Volume: 19 ISSN: 1107-3756 ISO Abbreviation: Int. J. Mol. Med. Publication Date: 2007 Mar |
Date Detail:
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Created Date: 2007-02-02 Completed Date: 2007-03-16 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9810955 Medline TA: Int J Mol Med Country: Greece |
Other Details:
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Languages: eng Pagination: 387-92 Citation Subset: IM |
Affiliation:
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Division of Hematology, Department of Internal Medicine, Institute for Advanced Medical Sciences, Hyogo College of Medicine, Hyogo 663-8501, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adipocytes
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cytology* Animals Antigens, CD34 / immunology Cell Differentiation* Cell Lineage Cell Proliferation Cell Transplantation Cells, Cultured Coculture Techniques Cytokines / genetics, metabolism Female Fetal Blood / cytology, transplantation Gene Expression Regulation Growth Substances / genetics, metabolism Hematopoietic Stem Cells / cytology* Humans Mesenchymal Stem Cells / cytology* Mice Mice, SCID RNA, Messenger / genetics, metabolism Severe Combined Immunodeficiency / pathology* Transplantation, Heterologous |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD34; 0/Cytokines; 0/Growth Substances; 0/RNA, Messenger |
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