| Adhesive interactions between fibroblasts and polymorphonuclear neutrophils in vitro. | |
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MedLine Citation:
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PMID: 1879435 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Although the in vivo interaction between polymorphonuclear neutrophils (PMN) and fibroblasts may be important, these pathways have not been well studied. We have investigated the adherence of PMN to monolayers of human fetal lung fibroblasts, using a microtiter plate assay based upon the uptake by cells of the vital stain Rose Bengal. Stimulation with phorbol myristate acetate (PMA) caused a significant increase of adherence over basal levels which was rapid in onset and plateaued at 5 min. Adhesion was dependent on the leucocyte integrin family of glycoproteins, notably on Mac-1, since monoclonal antibodies toward the beta chain (CD18) and alpha chain (CD11b) of Mac-1 almost completely suppressed PMA-induced PMN adhesion (88% and 77% inhibition, respectively). Adhesion was also inhibited by the peptides RGDS and GRGDS (24.2% and 26.6%, respectively using 1 mM peptide). Prestimulation of fibroblasts for longer time periods (5 and 24 h) with interleukin 1 alpha and tumor necrosis factor alpha, but not transforming growth factor beta, also resulted in a significant increase in adhesion of unstimulated PMN (after 24 h preincubation, 10 U/ml IL1 alpha stimulated adhesion by 179% of control, 500 U/ml TNF alpha by 157%). This indicated that there are both PMN- and fibroblast-dependent pathways for PMN adhesion. Components of the extracellular matrix of fibroblasts do not appear to play important roles in the adhesion process since addition of fibronectin and type IV collagen, or of purified antibodies to fibronectin and types I and IV collagen, did not affect PMA-induced PMN adhesion.(ABSTRACT TRUNCATED AT 250 WORDS) |
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Authors:
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A Shock; G J Laurent |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: European journal of cell biology Volume: 54 ISSN: 0171-9335 ISO Abbreviation: Eur. J. Cell Biol. Publication Date: 1991 Apr |
Date Detail:
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Created Date: 1991-10-03 Completed Date: 1991-10-03 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7906240 Medline TA: Eur J Cell Biol Country: GERMANY |
Other Details:
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Languages: eng Pagination: 211-6 Citation Subset: IM |
Affiliation:
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Department of Thoracic Medicine, National Heart and Lung Institute, University of London, United Kingdom. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Antibodies, Monoclonal Cell Adhesion / drug effects, physiology* Cell Adhesion Molecules / physiology Cells, Cultured Fibroblasts / drug effects, physiology* Humans Integrins / physiology Interleukin-1 / pharmacology Kinetics Lung / cytology Molecular Sequence Data Neuraminidase / metabolism Neutrophils / drug effects, physiology* Tetradecanoylphorbol Acetate / pharmacology Transforming Growth Factor beta / pharmacology Tumor Necrosis Factor-alpha / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Cell Adhesion Molecules; 0/Integrins; 0/Interleukin-1; 0/Transforming Growth Factor beta; 0/Tumor Necrosis Factor-alpha; 16561-29-8/Tetradecanoylphorbol Acetate; EC 3.2.1.18/Neuraminidase |
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