Document Detail


Adhesion to and invasion of cultured tick (Acarina: Ixodidae) cells by Borrelia burgdorferi (Spirochaetales: Spirochaetaceae) and maintenance of infectivity.
MedLine Citation:
PMID:  8510118     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lyme disease spirochetes, Borrelia burgdorferi, interact with cultured tick cells in ways similar to those reported to occur in the vector Ixodes dammini Spielman, Clifford, Piesman & Corwin. Spirochete adhesion and penetration were examined using a cell line from embryos of Rhipicephalus appendiculatus Neumann that morphologically resembles tick gut cells, RAE25. Cocultivation of B. burgdorferi with these cells permitted prolonged maintenance of infectivity for hamsters. Borrelial adherence to RAE25 cells was time- and density-dependent and increased by 10-15% per h during the first 5.5 h of cocultivation when we used a concentration of 4 x 10(7) spirochetes/ml. After 6 h, > 90% of the cells bound an average of 3-5 spirochetes per cell. Low passage, hamster-infective strains of B. burgdorferi (JMNT and CD16) showed a 2-3-fold higher rate of adhesion to RAE25 cells than the highly passaged, noninfectious strain B31. Inactivation of CD16 or JMNT by heat, starvation, or treatment with puromycin reduced adherence by 40-60%, whereas pretreatment with monoclonal antibodies to the outer surface proteins had no effect. Spirochetes adhered to young I. dammini cell lines to a similar degree as they did to RAE25, whereas lines from the ticks Dermacentor variabilis (Say) (RML15) and Boophilus microplus (Canestrini) (BME26) bound 30-60% fewer spirochetes. Electron microscopy revealed epicellular borreliae associated with coated pits and vesicles before endocytosis, and intracellular spirochetes were surrounded by a host cell-derived membrane.
Authors:
T J Kurtti; U G Munderloh; D E Krueger; R C Johnson; T G Schwan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of medical entomology     Volume:  30     ISSN:  0022-2585     ISO Abbreviation:  J. Med. Entomol.     Publication Date:  1993 May 
Date Detail:
Created Date:  1993-07-15     Completed Date:  1993-07-15     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0375400     Medline TA:  J Med Entomol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  586-96     Citation Subset:  IM    
Affiliation:
Department of Entomology, University of Minnesota, St. Paul 55108.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacterial Adhesion*
Borrelia burgdorferi Group / pathogenicity*
Cell Line / ultrastructure
Cricetinae
Microscopy, Electron
Ticks / microbiology*,  ultrastructure
Grant Support
ID/Acronym/Agency:
AR37909/AR/NIAMS NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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