Document Detail


Adhesion and stability of fibronectin on PTFE before and after seeding with normal and synchronized endothelial cells: in vitro study.
MedLine Citation:
PMID:  7763194     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The possible influence of the cell cycle on the efficacy of endothelial cell (EC) seeding onto the surface of polytetrafluoroethylene (PTFE) prostheses was studied. Likewise the ideal fibronectin concentration and optimal incubation time to guarantee the binding of this protein to the prosthetic surface have been calculated. Synchronized ECs, previously labeled with 3H-thymidine, were used, and the loss of radioactivity was determined at several times throughout the study. The results showed a progressive loss of cells on the prosthetic surface similar to that occurring with the seeding of unsynchronized ECs. The optimal concentration of fibronectin was 20 micrograms/ml, and the optimal incubation time was 1 h.
Authors:
N G Honduvilla; J Buján; M A Lizarbe; J M Bellón; N Olmo; A Hernando
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Artificial organs     Volume:  19     ISSN:  0160-564X     ISO Abbreviation:  Artif Organs     Publication Date:  1995 Feb 
Date Detail:
Created Date:  1995-06-29     Completed Date:  1995-06-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7802778     Medline TA:  Artif Organs     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  144-53     Citation Subset:  IM    
Affiliation:
Department of Morphological Sciences and Surgery, Faculty of Medicine, University of Alcalá de Henares, Spain.
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MeSH Terms
Descriptor/Qualifier:
Adsorption
Blood Vessel Prosthesis*
Cell Adhesion / physiology*
Cell Cycle / physiology*
Endothelium, Vascular / cytology*,  growth & development,  metabolism
Fibronectins / drug effects,  metabolism*
Glutaral / pharmacology
Humans
Polytetrafluoroethylene / metabolism*
Chemical
Reg. No./Substance:
0/Fibronectins; 111-30-8/Glutaral; 9002-84-0/Polytetrafluoroethylene

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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