Document Detail


Adhesion protein ApfA of Actinobacillus pleuropneumoniae is required for pathogenesis and is a potential target for vaccine development.
MedLine Citation:
PMID:  23269417     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Actinobacillus pleuropneumoniae is the etiologic agent of porcine pleuropneumonia, which causes serious economic losses in the pig farming industry worldwide. Due to a lack of knowledge of its virulence factors and a lack of effective vaccines able to confer cross-serotype protection, it is difficult to place this disease under control. By analyzing its genome sequences, we found that type IV fimbrial subunit protein ApfA is highly conserved among different serotypes of A. pleuropneumoniae. Our study shows that ApfA is an adhesin since its expression was greatly upregulated (135-fold) upon contact with host cells, while its deletion mutant attenuated its capability of adhesion. The inactivation of apfA dramatically reduced the ability of A. pleuropneumoniae to colonize mouse lung, suggesting that apfA is a virulence factor. Purified recombinant ApfA elicited an elevated humoral immune response and conferred robust protection against challenges with A. pleuropneumoniae serovar 1 strain 4074 and serovar 7 strain WF83 in mice. Importantly, the anti-ApfA serum conferred significant protection against both serovar 1 and serovar 7 in mice. These studies indicate that ApfA promotes virulence through attachment to host cells, and its immunogenicity renders it a promising novel subunit vaccine candidate against infection with A. pleuropneumoniae.
Authors:
Yang Zhou; Lu Li; Zhaohui Chen; Hong Yuan; Huanchun Chen; Rui Zhou
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-12-26
Journal Detail:
Title:  Clinical and vaccine immunology : CVI     Volume:  20     ISSN:  1556-679X     ISO Abbreviation:  Clin. Vaccine Immunol.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-01-29     Completed Date:  2013-07-09     Revised Date:  2013-08-09    
Medline Journal Info:
Nlm Unique ID:  101252125     Medline TA:  Clin Vaccine Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  287-94     Citation Subset:  IM    
Affiliation:
Division of Animal Infectious Diseases in the State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
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MeSH Terms
Descriptor/Qualifier:
Actinobacillus Infections / immunology*,  prevention & control
Actinobacillus pleuropneumoniae* / genetics,  immunology,  pathogenicity
Adhesins, Bacterial / genetics*,  immunology*
Animals
Antibodies, Bacterial
Bacterial Adhesion / genetics
Bacterial Vaccines / genetics,  immunology
Fimbriae, Bacterial / genetics,  immunology
Lung / microbiology
Mice
Pleuropneumonia / immunology,  microbiology,  prevention & control
Swine
Swine Diseases / genetics,  immunology,  prevention & control
Chemical
Reg. No./Substance:
0/Adhesins, Bacterial; 0/Antibodies, Bacterial; 0/Bacterial Vaccines
Comments/Corrections

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