Document Detail

Adhesion of pancreatic beta cells to biopolymer films.
MedLine Citation:
PMID:  19353639     Owner:  NLM     Status:  MEDLINE    
Dramatic reversal of Type 1 diabetes in patients receiving pancreatic islet transplants continues to prompt vigorous research concerning the basic mechanisms underlying patient turnaround. At the most fundamental level, transplanted islets must maintain viability and function in vitro and in vivo and should be protected from host immune rejection. Our previous reports showed enhancement of islet viability and insulin secretion per tissue mass for small islets (<125 mum) as compared with large islets (>125 mum), thus, demonstrating the effect of enhancing the mass transport of islets (i.e. increasing tissue surface area to volume ratio). Here, we report the facile dispersion of rat islets into individual cells that are layered onto the surface of a biopolymer film towards the ultimate goal of improving mass transport in islet tissue. The tightly packed structure of intact islets was disrupted by incubating in calcium-free media resulting in fragmented islets, which were further dispersed into individual or small groups of cells by using a low concentration of papain. The dispersed cells were screened for adhesion to a range of biopolymers and the nature of cell adhesion was characterized for selected groups by quantifying adherent cells, measuring the surface area coverage of the cells, and immunolabeling cells for adhesion proteins interacting with selected biopolymers. Finally, beta cells in suspension were centrifuged to form controlled numbers of cell layers on films for future work determining the mass transport limitations in the adhered tissue constructs. (c) 2009 Wiley Periodicals, Inc. Biopolymers 91: 676-685, 2009.This article was originally published online as an accepted preprint. The "Published Online" date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at
S Janette Williams; Qun Wang; Ronal R Macgregor; Teruna J Siahaan; Lisa Stehno-Bittel; Cory Berkland
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biopolymers     Volume:  91     ISSN:  0006-3525     ISO Abbreviation:  Biopolymers     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-06-03     Completed Date:  2009-09-02     Revised Date:  2014-09-21    
Medline Journal Info:
Nlm Unique ID:  0372525     Medline TA:  Biopolymers     Country:  United States    
Other Details:
Languages:  eng     Pagination:  676-85     Citation Subset:  IM    
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MeSH Terms
Cell Adhesion
Cell Survival
Coated Materials, Biocompatible
Insulin-Secreting Cells / cytology*,  physiology
Islets of Langerhans Transplantation
Materials Testing
Rats, Sprague-Dawley
Grant Support
1R03 AR054035-01A1/AR/NIAMS NIH HHS; P20 RR015563/RR/NCRR NIH HHS; P20 RR016443/RR/NCRR NIH HHS; R03 AR054035/AR/NIAMS NIH HHS; R03 AR054035-01A1/AR/NIAMS NIH HHS
Reg. No./Substance:
0/Biopolymers; 0/Coated Materials, Biocompatible

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