Document Detail


Adhesion and cellsurface properties of wild species of spore formers against enteric pathogens.
MedLine Citation:
PMID:  23339911     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
OBJECTIVE: To investigate the adhesion potential and cell surface properties against enteric pathogens Salmonella typhi, Salmonella para typhi A and Vibrio cholera.
METHODS: Adhesion potentials of spore and vegetative phase were studied separately for the isolates. Hydrophobic nature was measured on the basis of affinity towards the xylene. Autoaggregation and coaggregation were studied on the basis of clumping of cells. In vitro adhesion studies were done on mucous which were prepared from infant child faeces. Biofilm production of superior adhesive isolate was confirmed by SEM analysis.
RESULTS: Spore and vegetative phases of isolates possessed a different rate of adhesion potentials on intestinal mucous, which indicated that cell surface properties were involved in adhesion process. Spores showed a higher hydrophobicity than their vegetative cells which remained less or non hydrophobic. Vegetative phases showed capabilities for autoaggregation and coaggregation. Spores were found to be more adhesive on intestinal mucous than vegetative phase. Among enteric pathogens Vibrio cholera registered higher adhesion potentials with supporting cell surface properties. Among the five sporeforming isolates, isolate BM-3 possess superior adhesion than enteric pathogens and also exhibited biofilm formation which enhances colonization potential.
CONCLUSIONS: Spore and vegetative cell phases shows differences in adhesion potentials. Cell surface properties and adhesion studies reveals that isolate BM-3 can be selected as superior isolate which is capable for biofilm production. In short, isolate BM-3 possesses an enhanced adhesion potential than enteric pathogens towards intestinal mucous which is a desirable probiotic character.
Authors:
Anu P Sebastian; T R Keerthi
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Asian Pacific journal of tropical medicine     Volume:  6     ISSN:  1995-7645     ISO Abbreviation:  Asian Pac J Trop Med     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-01-23     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101533720     Medline TA:  Asian Pac J Trop Med     Country:  Singapore    
Other Details:
Languages:  eng     Pagination:  110-4     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 Hainan Medical College. Published by Elsevier B.V. All rights reserved.
Affiliation:
Microbial Biotechnology Lab, School of Biosciences, Mahatma Gandhi University, Kottayam- 686560, India.
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