Document Detail


Adhesion of Candida albicans to HeLa cells: studies using polystyrene beads.
MedLine Citation:
PMID:  17329863     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The adherence to HeLa cells by the yeast-type cells of the Candida albicans NIH A-207 strain cultivated for 2 d at 27 degrees C in the yeast extract-added Sabouraud liquid medium (YSLM) and the 500 mM galactose-added yeast nitrogen base medium (YNB-Gal) was compared. The yeast cells cultured in the YNB-Gal clearly increased the adherence to the HeLa cells compared to the YSLM. The adherence drastically decreased by pronase treatment of the yeast cells. Next, to define the sugar receptors of the yeast cells, lactosylceramide (LC)-, the H type 1 antigen (HA)-, Lewis(a) antigen (Le(a))-, mannan- and BSA-coated polystyrene beads were used for the adherence to the yeast cells. Only the LC- and HA-beads were bound to the yeast cells. The adherence of the two beads to the yeast cells cultured in the YNB-Gal was higher than that to the yeast cells cultured in the YSLM. The yeast cell wall mannan-coated polystyrene beads did not adhere at all to the Hela cells. Based on these results, it is evident that the protein parts involving the LC and HA receptors on the surface of the yeast cells correlate with the adherence to the HeLa cells.
Authors:
Masayoshi Miyauchi; Philippe Giummelly; Shin Yazawa; Yoshio Okawa
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biological & pharmaceutical bulletin     Volume:  30     ISSN:  0918-6158     ISO Abbreviation:  Biol. Pharm. Bull.     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-03-01     Completed Date:  2007-08-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9311984     Medline TA:  Biol Pharm Bull     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  588-90     Citation Subset:  IM    
Affiliation:
Department of Infection and Host Defense, Tohoku Pharmaceutical University, 4-4-1 Komatsushima, Aoba-ku, Sendai, Miyagi 981-8558, Japan.
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MeSH Terms
Descriptor/Qualifier:
Antigens, CD / chemistry,  metabolism
Antigens, Differentiation / chemistry,  metabolism
Candida albicans / cytology,  metabolism*
Cell Adhesion / drug effects
Epithelial Cells / metabolism,  pathology
Galactose / chemistry,  metabolism
Hela Cells
Humans
Lactosylceramides / chemistry,  metabolism
Lewis Blood-Group System / chemistry,  metabolism
Membrane Glycoproteins / chemistry
Microspheres*
Polystyrenes / chemistry*,  metabolism
Pronase / chemistry,  metabolism
Serum Albumin / chemistry,  metabolism
Chemical
Reg. No./Substance:
0/Antigens, CD; 0/Antigens, Differentiation; 0/H-type-1 antigen; 0/Lactosylceramides; 0/Lewis Blood-Group System; 0/Lewis Y antigen; 0/Membrane Glycoproteins; 0/Polystyrenes; 0/Serum Albumin; 0/mannoproteins; 26566-61-0/Galactose; 4682-48-8/CDw17 antigen; EC 3.4.24.-/Pronase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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