| Adherent cells generated during long-term culture of human umbilical cord blood CD34+ cells have characteristics of endothelial cells and beneficial effect on cord blood ex vivo expansion. | |
| | |
MedLine Citation:
|
PMID: 12634419 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Hematopoiesis depends on the association of hematopoietic stem cells with stromal cells that constitute the hematopoietic microenvironment. The in vitro development of the endothelial cell from umbilical cord blood (UCB) is not well established and has met very limited success. In this study, UCB CD34(+) cells were cultured for 5 weeks in a stroma-free liquid culture system using thrombopoietin, flt3 ligand, and granulocyte-colony stimulating factor. By week 4-5, we found that firmly adherent fibroblast-like cells were established. These cells showed characteristics of endothelial cells expressing von Willebrand factor, human vascular cell adhesion molecule-1, human intracellular adhesion molecule-1, human CD31, E-selectin, and human macrophage. Furthermore, when comparing an ex vivo system without an established endothelial monolayer to an ex vivo system with an established endothelial monolayer, better expansion of total nucleated cells, CD34(+) cells, and colony-forming units (CFUs)-granulocyte-macrophage and CFUs-granulocyte-erythroid-megakaryocyte-macrophage were found during culture. This phenomenon was in part due to the fact that a significant reduction of apoptotic fractions was found in the CD34(+) cells, which were cultured on the adherent monolayer for up to 5 weeks. To gather quantitative data on the number of endothelial cells derived from a given number of CD34 cells, we performed limiting dilution assay by using Poisson distribution: the number of tested cells (linear scale) producing a 37% negative culture (logarithmic scale) is the number of cells containing one endothelial cell. By this method, one endothelial cell may be found from 314 CD34(+) cells after 5 weeks of culture. These results suggest that the UCB CD34(+) cell fraction contains endothelial cell precursors, establishing the hematopoietic microenvironment and providing the beneficial effects through downregulating apoptosis on UCB expansion protocols. These observations may provide insight for future cellular therapy or graft engineering. |
| | |
Authors:
|
Eun-Sun Yoo; Kyung-Eun Lee; Jeong-Wan Seo; Eun-Hee Yoo; Mi-Ae Lee; Seock-Ah Im; Yeung-Chul Mun; Soon-Nam Lee; Jung-Won Huh; Mi-Jung Kim; Duck-Yeon Jo; Jee-Young Ahn; Sun-Mee Lee; Wha-Soon Chung; Jae-Hong Kim; Chu-Myong Seong |
Related Documents
:
|
1626349 - Electronmicroscopic examination of white cell reduction by four white cell-reduction fi... 8187809 - Human leucocyte-endothelial interactions in peripheral arterial occlusive disease. 3879599 - Endothelial cells suppress granulocyte clusters and stimulate large granulocyte colonie... 6268539 - Deleterious effects of endotoxin on cultured endothelial cells: an in vitro model of va... 11311329 - Migration of human granulocytes through reconstituted basement membrane is not dependen... 23441799 - Adhesion of streptococci to various orthodontic composite resins. 17924279 - An in-vivo analysis of capillary stasis and endothelial apoptosis in a model of hyperte... 15707889 - Vessels and nerves: marching to the same tune. 23474289 - Roles of the epoxygenase cyp2j2 in the endothelium. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Stem cells (Dayton, Ohio) Volume: 21 ISSN: 1066-5099 ISO Abbreviation: Stem Cells Publication Date: 2003 |
Date Detail:
|
Created Date: 2003-03-13 Completed Date: 2003-09-29 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 9304532 Medline TA: Stem Cells Country: United States |
Other Details:
|
Languages: eng Pagination: 228-35 Citation Subset: IM |
Affiliation:
|
Department of Pediatrics, College of Medicine, Ewha Women's University, Seoul, Korea. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Antibodies Antigens, CD34 / analysis*, immunology Apoptosis Cell Separation Cells, Cultured Dactinomycin / analogs & derivatives* Down-Regulation Endothelium, Vascular / cytology* Fetal Blood / cytology* Fibroblasts / cytology Fluorescent Dyes Hematopoietic Stem Cells / chemistry*, cytology* Humans Intercellular Adhesion Molecule-1 / analysis, immunology Vascular Cell Adhesion Molecule-1 / analysis, immunology von Willebrand Factor / analysis, immunology |
| Chemical | |
Reg. No./Substance:
|
0/Antibodies; 0/Antigens, CD34; 0/Fluorescent Dyes; 0/Vascular Cell Adhesion Molecule-1; 0/von Willebrand Factor; 126547-89-5/Intercellular Adhesion Molecule-1; 50-76-0/Dactinomycin; 7240-37-1/7-aminoactinomycin D |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Human umbilical cord blood as a source of transplantable hepatic progenitor cells.
Next Document: Interleukin-3 promotes proliferation and differentiation of human hematopoietic stem cells but reduc...