Document Detail


Adherence of uropathogenic E. coli to differentiated human uroepithelial cells grown in vitro.
MedLine Citation:
PMID:  2104636     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A quantitative in vitro model to measure E. coli adherence to differentiated human uroepithelial cells has been developed. Primary cultures of uroepithelial cells were initiated from normal ureteral epithelium. Adherence of uropathogenic 3H-labelled Escherichia coli to postconfluent human uroepithelial cells was directly related to the bacteria:epithelial cell ratio during incubation. Bacterial attachment was inhibited either by mannose or by urine containing anti-E. coli antibodies. Transmission electron microscopy showed that epithelial cells differentiated in vitro to resemble normal uroepithelium in vivo. Furthermore, electron microscopy showed specific adherence of bacteria to the glycocalyx of microvilli of the superficial uroepithelial cells in vitro in a manner which closely mimics the in vivo interaction. This model of bacterial adherence permits in vitro analysis of adhesin-receptor interactions between uropathogenic E. coli and a layer of viable uroepithelial cells similar to those lining the bladder.
Authors:
W J Hopkins; C A Reznikoff; T D Oberley; D T Uehling
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of urology     Volume:  143     ISSN:  0022-5347     ISO Abbreviation:  J. Urol.     Publication Date:  1990 Jan 
Date Detail:
Created Date:  1990-02-05     Completed Date:  1990-02-05     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0376374     Medline TA:  J Urol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  146-9     Citation Subset:  AIM; IM    
Affiliation:
Department of Surgery, Wisconsin Clinical Cancer Center, Madison.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacterial Adhesion* / drug effects
Cells, Cultured
Epithelium / microbiology,  ultrastructure
Escherichia coli / immunology,  pathogenicity,  physiology*
Haplorhini
Humans
Immunization
Immunoglobulins / urine
Mannose / pharmacology
Ureter / microbiology*,  ultrastructure
Grant Support
ID/Acronym/Agency:
AM30808/AM/NIADDK NIH HHS
Chemical
Reg. No./Substance:
0/Immunoglobulins; 31103-86-3/Mannose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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