Document Detail


Adherence of skin bacteria to human epithelial cells.
MedLine Citation:
PMID:  2298877     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Aerobic and anaerobic bacteria isolated from human axillae were tested for their capacity to adhere to buccal epithelial cells, immortalized human epithelial (HEp-2) cells, and undifferentiated and differentiated human epithelial cells. In general, both aerobic and anaerobic diphtheroids adhered better to differentiated human epithelial cells than to HEp-2 and undifferentiated human epithelial cells (P less than 0.05). Mannose, galactose, fucose, N-acetyl-D-glucosamine, and fibronectin were also assayed for their capacity to inhibit the adherence of diphtheroids to human epithelial cells. A great deal of variability was observed in the capacity of the latter compounds to inhibit the attachment of aerobic diphtheroids to undifferentiated and differentiated epithelial cells. Overall, mannose appeared to be best at inhibiting the adherence of the aerobic diphtheroids to undifferentiated human epithelial cells. Galactose, fucose, N-acetyl-D-glucosamine, and fibronectin showed a greater capacity to inhibit attachment of aerobic diphtheroids to differentiated than to undifferentiated human epithelial cells. The inhibition of adherence to differentiated human epithelial cells varied with the microorganism and the compound tested; however, the highest and most consistent inhibition of adherence (76.1 to 88.6%) was observed with a 5% solution of N-acetyl-D-glucosamine. The in vitro adherence and adherence inhibition assays presented here demonstrate that a number of adhesins and receptors are involved in the adherence of skin bacteria to human epithelial cells and receptors on human epithelial cells are apparently altered during differentiation.
Authors:
S Romero-Steiner; T Witek; E Balish
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  28     ISSN:  0095-1137     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  1990 Jan 
Date Detail:
Created Date:  1990-03-07     Completed Date:  1990-03-07     Revised Date:  2010-09-10    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  27-31     Citation Subset:  IM    
Affiliation:
Department of Medical Microbiology, University of Wisconsin Medical School, Madison 53706.
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MeSH Terms
Descriptor/Qualifier:
Bacteria, Aerobic / isolation & purification
Bacteria, Anaerobic / isolation & purification
Bacterial Adhesion*
Cell Differentiation
Cell Line
Corynebacterium / isolation & purification
Epithelial Cells
Epithelium / microbiology
Female
Humans
Male
Mouth Mucosa / cytology,  microbiology
Skin / cytology,  microbiology*
Comments/Corrections

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