Document Detail


Adherence of peritonitis-causing staphylococci to human peritoneal mesothelial cell monolayers.
MedLine Citation:
PMID:  2299208     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The adherence of staphylococci to monolayers of human mesothelial cells was studied. Adherence of Staphylococcus aureus to mesothelial cell monolayers was 3.4-fold better than to plastic (P less than .01) whereas that of Staphylococcus epidermidis was 3.0-fold less than to plastic (P less than .01). Neither serum albumin nor gelatin inhibited staphylococcal binding. S. aureus adherence correlated with the amount of cell wall protein A (r = .63, P less than .05) but not with fibronectin binding; it was significantly inhibited by the addition of purified cell wall lipoteichoic acid (55% +/- 2.7%), teichoic acid (34.5% +/- 3.4%), and protein A (25.6% +/- 2.9%) but not peptidoglycan. Protein A- and teichoic acid-deficient mutants adhered less well than their parent strains, and encapsulated S. epidermidis adhere well to human monothelial cells. Staphylococcal binding may involve cell wall lipoteichoic acid, teichoic acid, and protein A.
Authors:
I A Haagen; H C Heezius; R P Verkooyen; J Verhoef; H A Verbrugh
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of infectious diseases     Volume:  161     ISSN:  0022-1899     ISO Abbreviation:  J. Infect. Dis.     Publication Date:  1990 Feb 
Date Detail:
Created Date:  1990-03-09     Completed Date:  1990-03-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0413675     Medline TA:  J Infect Dis     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  266-73     Citation Subset:  AIM; IM    
Affiliation:
Laboratory of Microbiology, University of Utrecht Medical School, The Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Adhesion*
Blood Proteins / metabolism
Cell Wall / metabolism
Cells, Cultured
Humans
Lipopolysaccharides / metabolism
Peptidoglycan / metabolism
Peritoneum / microbiology*
Peritonitis / microbiology*
Staphylococcal Protein A / metabolism
Staphylococcus aureus / metabolism*,  ultrastructure
Staphylococcus epidermidis / metabolism*,  ultrastructure
Teichoic Acids / metabolism
Chemical
Reg. No./Substance:
0/Blood Proteins; 0/Lipopolysaccharides; 0/Peptidoglycan; 0/Staphylococcal Protein A; 0/Teichoic Acids; 56411-57-5/lipoteichoic acid

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