Document Detail


Adherence of Brucella to human epithelial cells and macrophages is mediated by sialic acid residues.
MedLine Citation:
PMID:  15056214     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The basis for the interaction of Brucella species with the surface of epithelial cells before migration in the host within polymorphonuclear leucocytes is largely unknown. Here, we studied the ability of Brucella abortus and Brucella melitensis to adhere to cultured epithelial (HeLa and HEp-2) cells and THP-1-derived macrophages, and to bind extracellular matrix proteins (ECM). The brucellae adhered to epithelial cells forming localized bacterial microcolonies on the cell surface, and this process was inhibited significantly by pretreatment of epithelial cells with neuraminidase and sodium periodate and by preincubation of the bacteria with heparan sulphate and N-acetylneuraminic acid. Trypsinization of epithelial cells yielded increased adherence, suggesting unmasking of target sites on host cells. Notably, the brucellae also adhered to cultured THP-1 cells, and this event was greatly reduced upon removal of sialic acid residues from these cells with neuraminidase. B. abortus bound in a dose-dependent manner to immobilized fibronectin and vitronectin and, to a lesser extent, to chondroitin sulphate, collagen and laminin. In sum, our data strongly suggest that the adherence mechanism of brucellae to epithelial cells and macrophages is mediated by cellular receptors containing sialic acid and sulphated residues. The recognition of ECM (fibronectin and vitronectin) by the brucellae may represent a mechanism for spread within the host tissues. These are novel findings that offer new insights into understanding the interplay between Brucella and host cells.
Authors:
Elsa I Castañeda-Roldán; Fabiola Avelino-Flores; Monique Dall'Agnol; Enrique Freer; Lilia Cedillo; Jacques Dornand; Jorge A Girón
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cellular microbiology     Volume:  6     ISSN:  1462-5814     ISO Abbreviation:  Cell. Microbiol.     Publication Date:  2004 May 
Date Detail:
Created Date:  2004-04-01     Completed Date:  2004-07-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100883691     Medline TA:  Cell Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  435-45     Citation Subset:  IM    
Affiliation:
Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Edificio 76, Complejo de Ciencias, Puebla, México.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacterial Adhesion / physiology*
Brucella abortus / physiology*,  ultrastructure
Brucella melitensis / metabolism*,  ultrastructure
Cell Line
Enzyme Inhibitors / metabolism
Epithelial Cells / metabolism,  microbiology*,  ultrastructure
Extracellular Matrix Proteins / chemistry,  metabolism
Humans
Macrophages / cytology,  metabolism*,  microbiology
N-Acetylneuraminic Acid / metabolism*
Neuraminidase / metabolism
Periodic Acid / metabolism
Trypsin / metabolism
Chemical
Reg. No./Substance:
0/Enzyme Inhibitors; 0/Extracellular Matrix Proteins; 10450-60-9/Periodic Acid; 131-48-6/N-Acetylneuraminic Acid; 7790-28-5/metaperiodate; EC 3.2.1.18/Neuraminidase; EC 3.4.21.4/Trypsin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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