Document Detail

Adenylyl cyclases in oocyte maturation: a characterization of AC isoforms in bovine cumulus cells.
MedLine Citation:
PMID:  16804883     Owner:  NLM     Status:  MEDLINE    
Mammalian oocytes are arrested at the G(2)/M transition in the meiotic cell cycle. It is well known that a decrease in intraoocyte cAMP concentrations accompanies resumption of meiosis, but the precise trigger of this decrease remains a mystery. Follicular somatic cells are intimately coupled to the oocyte and are thought to transmit maturation signals to the oocyte in response to hormonal stimulation. Here, we investigate the nature of the follicular somatic cell response to hormonal stimulation by identifying and characterizing the adenylate cyclase isoforms present in bovine cumulus cells. RT-PCR and Western blot analysis revealed the presence of multiple adenylyl cyclase isoforms in bovine granulosa and cumulus cells. Pharmacological manipulation of the AC isoforms showed that multiple isoforms were indeed active. Our data indicate that the PKC inhibited adenylate cyclases IV and VI and the calcium-stimulated isoform I predominate in bovine cumulus cells.
Michele Lastro; Sarah Collins; W Bruce Currie
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Molecular reproduction and development     Volume:  73     ISSN:  1040-452X     ISO Abbreviation:  Mol. Reprod. Dev.     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-08-07     Completed Date:  2007-02-09     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8903333     Medline TA:  Mol Reprod Dev     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1202-10     Citation Subset:  IM    
Department of Animal Science, Cornell University, Ithaca, New York 14853, USA.
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MeSH Terms
Adenylate Cyclase / genetics,  metabolism*
Calcium / metabolism
Cell Differentiation*
Cell Survival
Cells, Cultured
Cyclic AMP / metabolism
GTP-Binding Proteins / metabolism
Gene Expression Regulation, Enzymologic
Granulosa Cells / enzymology
Isoenzymes / genetics,  metabolism
Oocytes / cytology*,  enzymology*
Protein Kinases / metabolism
Transcription, Genetic / genetics
Reg. No./Substance:
0/Isoenzymes; 60-92-4/Cyclic AMP; 7440-70-2/Calcium; EC 2.7.-/Protein Kinases; EC 3.6.1.-/GTP-Binding Proteins; EC Cyclase

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