Document Detail


Adenylate gradients and Ar:O2 effects on legume nodules. II. Changes in the subcellular adenylate pools.
MedLine Citation:
PMID:  15064369     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Central infected zone tissue of soybean (Glycine max L. Merr.) nodules was fractionated into separate subcellular compartments using density gradient centrifugation in nonaqueous solvents to better understand how exposure to Ar:O(2) (80:20%, v/v) atmosphere affects C and N metabolism, and to explore a potential role for adenylates in regulating O(2) diffusion. When nodules were switched from air to Ar:O(2), adenylate energy charge (AEC) in the plant cytosol rose from 0.63 +/- 0.02 to 0.73 +/- 0.02 within 7 min and to 0.80 +/- 0.01 by 60 min. In contrast, AEC of the mitochondrial compartment of this central zone tissue remained high (0.80 +/- 0.02 to 0.81 +/- 0.02) following Ar treatment while that of the bacteroid compartment was unchanged, at 0.73 +/- 0.02, after 7 min, but declined to 0.57 +/- 0.03 after 60 min. These results were consistent with a simulation model that predicted Ar:O(2) exposure would first reduce ATP demand for ammonia assimilation and rapidly increase cytosolic AEC, before the Ar:O(2)-induced decline mediated by a decrease in nodule O(2) permeability reduces bacteroid AEC. The possibility that adenylates play a key, integrating role in regulating nodule permeability to oxygen diffusion is discussed.
Authors:
Hui Wei; Craig A Atkins; David B Layzell
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-04-02
Journal Detail:
Title:  Plant physiology     Volume:  134     ISSN:  0032-0889     ISO Abbreviation:  Plant Physiol.     Publication Date:  2004 Apr 
Date Detail:
Created Date:  2004-04-15     Completed Date:  2004-08-19     Revised Date:  2010-09-21    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1775-83     Citation Subset:  IM    
Affiliation:
Department of Biology, Queen's University, Kingston, Ontario, Canada K7L 3N6.
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MeSH Terms
Descriptor/Qualifier:
Adenosine Monophosphate / chemistry,  metabolism*
Adenosine Triphosphate / metabolism
Argon / pharmacology*
Bacteria / growth & development
Biological Transport / physiology
Cytosol / metabolism
Diffusion / drug effects
Fabaceae / drug effects,  growth & development*,  microbiology
Nitrogen / pharmacology
Nitrogenase / metabolism
Oxygen / pharmacology*
Oxygen Consumption / drug effects,  physiology
Chemical
Reg. No./Substance:
56-65-5/Adenosine Triphosphate; 61-19-8/Adenosine Monophosphate; 7440-37-1/Argon; 7727-37-9/Nitrogen; 7782-44-7/Oxygen; EC 1.18.6.1/Nitrogenase
Comments/Corrections

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