Document Detail


Adenylate cyclase activity of NIH 3T3 cells morphologically transformed by ras genes.
MedLine Citation:
PMID:  3081369     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The observed homology between G-proteins which regulate adenylate cyclase and ras proteins and the suggested role of ras in the regulation of adenylate cyclase in yeast prompted us to examine the regulation of adenylate cyclase in three cell lines: (i) NIH 3T3 cells, (ii) NIH 3T3 cells transformed by high levels of the normal rasH gene product and (iii) NIH 3T3 cells transformed by a mutated rasH gene product. We found that the regulation of adenylate cyclase by G-proteins is identical in the three cell lines, although the response of the transformed NIH 3T3 cells to agonists is strongly attenuated. Our data suggest that mammalian ras products do not interact directly with adenylate cyclase, although their increased expression may indirectly inhibit the interaction of adenylate cyclase stimulatory receptors with G-proteins.
Authors:
A Levitzki; J Rudick; I Pastan; W C Vass; D R Lowy
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  FEBS letters     Volume:  197     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1986 Mar 
Date Detail:
Created Date:  1986-04-24     Completed Date:  1986-04-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  134-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adenylate Cyclase / metabolism*
Alprostadil / pharmacology
Animals
Cell Line
Cell Transformation, Neoplastic*
Cell Transformation, Viral*
Enzyme Activation / drug effects
Forskolin / pharmacology
GTP-Binding Proteins / physiology
Harvey murine sarcoma virus
Isoproterenol / pharmacology
Mice
Oncogenes*
Chemical
Reg. No./Substance:
66428-89-5/Forskolin; 745-65-3/Alprostadil; 7683-59-2/Isoproterenol; EC 3.6.1.-/GTP-Binding Proteins; EC 4.6.1.1/Adenylate Cyclase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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