Document Detail


Adenovirus transduction of 3T3-L1 cells.
MedLine Citation:
PMID:  11254759     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
3T3-L1 cells offer an excellent model system for studies of differentiation and biochemistry of fat cells. However, these cells are limited in their utility by the low efficiency with which DNA can be introduced by transfection. Gene delivery by viral vectors, particularly adenovirus, has proven a powerful means for introduction of genes into certain cell types. Furthermore, adenovirus transduction has been used to study mechanisms involved in the differentiation of 3T3-L1 cells into mature fat cells. We show in this study that 3T3-L1 cells are inefficiently transduced by adenovirus. The potential advantages offered by adenovirus transduction led us to examine methods designed to enhance transduction of 3T3-L1 cells by adenovirus. Of these methods, polylysine-mediated enhancement demonstrates considerable promise because it permits up to 100% of cells to be transduced and because it does not inhibit differentiation of 3T3-L1 cells. -- Orlicky D. J., and J. Schaack. Adenovirus transduction of 3T3-L1 cells. J. Lipid Res. 2001. 42: 460--466.
Authors:
D J Orlicky; J Schaack
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of lipid research     Volume:  42     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  2001 Mar 
Date Detail:
Created Date:  2001-03-20     Completed Date:  2001-05-21     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  460-6     Citation Subset:  IM    
Affiliation:
Department of Pathology, University of Colorado Cancer Center, Denver, CO 80262, USA. David.Orlicky@UCHSC.edu
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MeSH Terms
Descriptor/Qualifier:
3T3 Cells
Adenoviridae / genetics*
Adipocytes / metabolism*
Animals
Cation Exchange Resins / pharmacology
Cell Differentiation
Cytomegalovirus / genetics
Fluorescence
Genetic Vectors*
Green Fluorescent Proteins
Indicators and Reagents
Lipid Metabolism
Lipids / pharmacology
Luminescent Proteins / genetics
Mice
Polylysine / pharmacology
Transfection*
Grant Support
ID/Acronym/Agency:
HL58344/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Cation Exchange Resins; 0/Indicators and Reagents; 0/Lipids; 0/Lipofectamine; 0/Luminescent Proteins; 147336-22-9/Green Fluorescent Proteins; 25104-18-1/Polylysine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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