| Adenovirus-mediated human prostasin gene delivery is linked to increased aldosterone production and hypertension in rats. | |
| | |
MedLine Citation:
|
PMID: 12626364 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Prostasin has been demonstrated to be an activator of epithelial sodium channels in cultured renal and bronchial epithelial cells. In this study, we evaluated the effects of adenovirus-mediated gene transfer of human prostasin on blood pressure regulation and sodium reabsorption in Wistar rats. Expression of human prostasin mRNA was identified in rat adrenal gland, liver, kidney, heart, lung, and aorta, and immunoreactive human prostasin was detected in the circulation and urine of rats receiving prostasin gene transfer. A single injection of adenovirus carrying the prostasin gene caused prolonged increases in blood pressure for 3-4 wk. Blood pressure increase was accompanied by elevated plasma aldosterone levels and reduced plasma renin activity. The increase in blood pressure and plasma aldosterone levels as well as the reduction of plasma renin activity correlated with the expression of human prostasin transgene. Elevated plasma aldosterone levels were detected at 3 days after gene transfer before the development of hypertension, indicating that stimulation of mineralocorticoid production is the primary target of prostasin. Prostasin gene transfer significantly reduced urinary K(+) excretion but increased urinary Na(+) and kallikrein excretion. Elevated renal kallikrein levels promote natriuresis, which may lead to sodium escape and prevent further increases of blood pressure after prostasin gene transfer. In summary, these results suggest that prostasin participates in blood pressure and electrolyte homeostasis by regulating the renin-angiotensin-aldosterone and kallikrein-kinin systems. |
| | |
Authors:
|
Cindy Wang; Julie Chao; Lee Chao |
Related Documents
:
|
3520094 - Blood pressure control in normotensive members of hypertensive families. 14551174 - Can renin status predict the antihypertensive efficacy of eplerenone add-on therapy? 9854594 - Essential hypertension: first reason for persistent hypertension after unilateral adren... 6375284 - Effects of verapamil in hypertensive patients. 12237804 - Cardiovascular effects of urtica dioica l. in isolated rat heart and aorta. 20124424 - Everyday discrimination and nocturnal blood pressure dipping in black and white americans. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. Date: 2002-12-19 |
Journal Detail:
|
Title: American journal of physiology. Regulatory, integrative and comparative physiology Volume: 284 ISSN: 0363-6119 ISO Abbreviation: Am. J. Physiol. Regul. Integr. Comp. Physiol. Publication Date: 2003 Apr |
Date Detail:
|
Created Date: 2003-03-10 Completed Date: 2003-04-17 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 100901230 Medline TA: Am J Physiol Regul Integr Comp Physiol Country: United States |
Other Details:
|
Languages: eng Pagination: R1031-6 Citation Subset: IM |
Affiliation:
|
Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, South Carolina 29425-2211, USA. wangxi@musc.edu |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adenoviridae
/
genetics* Aldosterone / blood, metabolism* Animals Blood Pressure Disease Models, Animal Female Gene Expression Genetic Vectors / genetics Humans Hypertension / blood, genetics, metabolism*, urine Male Potassium / urine RNA, Messenger / genetics, metabolism Rats Rats, Wistar Serine Endopeptidases / genetics*, metabolism* Time Factors Water-Electrolyte Balance |
| Grant Support | |
ID/Acronym/Agency:
|
HL-29397/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/RNA, Messenger; 52-39-1/Aldosterone; 7440-09-7/Potassium; EC 3.4.21.-/Serine Endopeptidases; EC 3.4.21.-/prostasin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Fos, RVLM-projecting neurons, and spinally projecting neurons in the PVN following hypertonic saline...
Next Document: Mechanisms regulating the marked seasonal variation in melatonin synthesis in the European hamster p...