Document Detail

Adenovirus-mediated high expression of BCL-6 in CV-1 cells induces apoptotic cell death accompanied by down-regulation of BCL-2 and BCL-X(L).
MedLine Citation:
PMID:  9927205     Owner:  NLM     Status:  MEDLINE    
The BCL-6 proto-oncogene encodes a 92- to 98-kDa transcriptional repressor containing the BTB/POZ domain at its N-terminal region and the zinc finger domain at its C-terminal region, respectively. In the present study, we examined the function of BCL-6 by using a recombinant adenovirus expressing BCL-6 (Ax1CA-BCL-6) and the lacZ reporter gene (Ax1CA-lacZ). Viability of CV-1 and HeLa cells infected with Ax1CA-BCL-6 was markedly reduced due to apoptosis, suggesting that BCL-6-overexpression induces apoptosis in CV-1 and HeLa cells. FACS analysis revealed that BCL-6-overexpressing cells are accumulated not only at the sub-G1 but also at G2/M phase. Induction of apoptosis by BCL-6 was preceded by down-regulation of apoptosis repressors BCL-2 and BCL-X(L). These results suggest that BCL-6 induces apoptosis by regulating the expression of these apoptosis-regulating genes.
T Yamochi; Y Kaneita; T Akiyama; S Mori; M Moriyama
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Oncogene     Volume:  18     ISSN:  0950-9232     ISO Abbreviation:  Oncogene     Publication Date:  1999 Jan 
Date Detail:
Created Date:  1999-02-16     Completed Date:  1999-02-16     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  8711562     Medline TA:  Oncogene     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  487-94     Citation Subset:  IM    
Department of Pathology, the Institute of Medical Science, the University of Tokyo, Japan.
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MeSH Terms
Adenoviridae / genetics*
Apoptosis / genetics*
Cell Cycle / genetics
Cell Line
DNA-Binding Proteins / genetics*
Hela Cells
Proto-Oncogene Proteins / genetics*
Proto-Oncogene Proteins c-bcl-2 / genetics*
Proto-Oncogene Proteins c-bcl-6
Recombination, Genetic
Transcription Factors / genetics*
Transduction, Genetic
bcl-X Protein
Reg. No./Substance:
0/BCL2L1 protein, human; 0/DNA-Binding Proteins; 0/Proto-Oncogene Proteins; 0/Proto-Oncogene Proteins c-bcl-2; 0/Proto-Oncogene Proteins c-bcl-6; 0/Transcription Factors; 0/bcl-X Protein

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