Document Detail


Adenovirus-mediated expression of a ribozyme to c-myb mRNA inhibits smooth muscle cell proliferation and neointima formation in vivo.
MedLine Citation:
PMID:  10438864     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Smooth muscle cell (SMC) proliferation is an important component of restenosis in response to injury after balloon angioplasty. Inhibition of proliferation in vivo can limit neointima hyperplasia in animal models of restenosis. Ribozymes against c-myb mRNA have been shown to be effective inhibitors of SMC proliferation in vitro. The effectiveness of adenovirus as a gene therapy vector in animal models of restenosis is well documented. In order to test the utility of ribozymes to inhibit SMC proliferation by a gene therapy approach, recombinant adenovirus expressing ribozymes against c-myb mRNA was generated and tested both in vitro and in vivo. This adenovirus ribozyme vector is shown to inhibit SMC proliferation in culture and neointima formation in a rat carotid artery balloon injury model of restenosis.
Authors:
D G Macejak; H Lin; S Webb; J Chase; K Jensen; T C Jarvis; J M Leiden; L Couture
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of virology     Volume:  73     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  1999 Sep 
Date Detail:
Created Date:  1999-09-07     Completed Date:  1999-09-07     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  7745-51     Citation Subset:  IM    
Affiliation:
Ribozyme Pharmaceuticals, Inc., Boulder, Colorado 80301, USA. macejad@rpi.com
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MeSH Terms
Descriptor/Qualifier:
Adenoviridae*
Angioplasty, Balloon
Animals
Cell Division
Cell Line
Cells, Cultured
Gene Expression
Genetic Vectors*
Humans
Mice
Muscle, Smooth, Vascular / cytology,  metabolism*
Proto-Oncogene Proteins / genetics,  metabolism*
Proto-Oncogene Proteins c-myb
RNA, Catalytic / genetics,  metabolism*
RNA, Messenger
Rats
Trans-Activators / genetics,  metabolism*
Chemical
Reg. No./Substance:
0/Proto-Oncogene Proteins; 0/Proto-Oncogene Proteins c-myb; 0/RNA, Catalytic; 0/RNA, Messenger; 0/Trans-Activators
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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