| Adenovirus-mediated acidic fibroblast growth factor gene transfer induces angiogenesis in the nonischemic rabbit heart. | |
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MedLine Citation:
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PMID: 10527767 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Most patients with severe coronary artery disease have normal baseline myocardial blood flow. Therefore, interventions aimed at inducing therapeutic angiogenesis in these patients should cause new blood vessel growth in the heart in the absence of chronic ischemia. It was examined whether adenovirus-mediated gene transfer of recombinant, secreted acidic fibroblast growth factor (sp+aFGF(1-154)), next to a major epicardial artery, may induce neovascularization and reduce the risk region for myocardial infarction upon coronary ligation near the injection site. Fifteen days prior to coronary artery occlusion, rabbits were treated with intramyocardial injections of AdCMV.sp+aFGF(1-154), the control vector AdCMV.NLSbetagal (1 x 10(9) plaque-forming units), or saline. Messenger RNA transcripts for aFGF(1-154) were present up to 12 days after injection in the tissues exposed to AdCMV.aFGF(1-154). Following coronary artery occlusion rabbits treated with AdCMV. sp+aFGF(1-154) showed a 50% reduction of the risk region for myocardial infarction (P < 0.01 vs control). Histologic analysis showed a twofold increase in length density of intramural coronary arterioles (P < 0.01 vs control) and a 17% increase in length density of the capillary network (P < 0.001) in the myocardium exposed to AdCMV.sp+aFGF(1-154). Thus, gene therapy with AdCMV. sp+aFGF(1-154) can induce angiogenesis in the absence of chronic ischemia. The newly formed collateral blood vessels provide an anatomical basis for the reduction in the risk region for myocardial infarction upon subsequent occlusion of the coronary artery in proximity of the site where angiogenesis was induced. |
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Authors:
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J Safi; A F DiPaula; T Riccioni; J Kajstura; G Ambrosio; L C Becker; P Anversa; M C Capogrossi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Microvascular research Volume: 58 ISSN: 0026-2862 ISO Abbreviation: Microvasc. Res. Publication Date: 1999 Nov |
Date Detail:
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Created Date: 1999-12-10 Completed Date: 1999-12-10 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0165035 Medline TA: Microvasc Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 238-49 Citation Subset: IM |
Copyright Information:
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Copyright 1999 Academic Press. |
Affiliation:
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Laboratory of Cardiovascular Science, National Institutes of Health, Baltimore, Maryland 21224, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenoviridae
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genetics* Animals Collateral Circulation / genetics Coronary Disease / pathology, therapy Disease Models, Animal Fibroblast Growth Factor 1 / genetics*, physiology Gene Expression Gene Transfer Techniques* Genetic Vectors Humans Male Myocardial Infarction / prevention & control Neovascularization, Physiologic / genetics* RNA, Messenger / genetics, metabolism Rabbits |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; 104781-85-3/Fibroblast Growth Factor 1 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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