Document Detail


Adenovirus E1A 12S protein induces DNA synthesis and proliferation in primary epithelial cells in both the presence and absence of serum.
MedLine Citation:
PMID:  3027395     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Infection of primary baby rat kidney (BRK) cells with an adenovirus that carries an E1A 12S cDNA in place of the normal E1A region (adenovirus 5 [Ad5] 12S) resulted in the induction of cellular DNA synthesis and proliferation of the epithelial cells in the population, even in the absence of serum. Increased cellular DNA synthesis was first detectable by 12 h after infection and was maintained at a 10- to 20-fold higher level than in mock-infected cells. By 5 days after infection there was a 10-fold-greater number of 12S virus-infected BRK cells. These infected BRK cells retained many of their normal epithelial cell characteristics and were not transformed. The expression of the E1A 12S protein(s) occurred early after infection. There was no induction of adenoviral gene expression or viral DNA replication in these cells. The early effects of a fully transforming gene product(s) were also examined. The Ad5-simian virus 40 hybrid virus, Ad5.SVR4, in which the early region of simian virus 40 has replaced the E1 region of Ad5, was used to infect BRK cells. The kinetics of expression of the T antigens were similar to those of the 12S polypeptides. Infection with Ad5.SV4 also resulted in the induction of cellular DNA synthesis and cell proliferation at levels similar to those observed with the 12S virus. However, infection with Ad5.SVR4 resulted in cells that had lost some of their epithelial cell characteristics and were fully transformed. Thus, although the early cellular events induced by the two genes were similar, they did not yield the same final cellular phenotype.
Authors:
M P Quinlan; T Grodzicker
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of virology     Volume:  61     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  1987 Mar 
Date Detail:
Created Date:  1987-03-24     Completed Date:  1987-03-24     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  673-82     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adenovirus Early Proteins
Adenoviruses, Human / genetics*
Antigens, Viral, Tumor / genetics
Cell Cycle*
Culture Media
DNA / biosynthesis*
DNA, Viral / biosynthesis
Epithelial Cells
Gene Expression Regulation
Growth Substances / blood
Mutation
Oncogene Proteins, Viral / genetics*
Simian virus 40
Grant Support
ID/Acronym/Agency:
CA 13106/CA/NCI NIH HHS; F32CA97676/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Adenovirus Early Proteins; 0/Antigens, Viral, Tumor; 0/Culture Media; 0/DNA, Viral; 0/Growth Substances; 0/Oncogene Proteins, Viral; 9007-49-2/DNA
Comments/Corrections

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