Document Detail


Adenoviral-mediated endothelial precursor cell delivery of soluble CD115 suppresses human prostate cancer xenograft growth in mice.
MedLine Citation:
PMID:  19522014     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Prostate cancer tumor growth and neovascularization is promoted by an interplay between migratory tumor stromal cells such as specialized tumor-associated macrophages (TAMs) and circulating endothelial precursor cells (CEPs). As vehicles for tumor therapy, human CEPs are relatively easy to isolate from peripheral blood, are able to proliferate long-term in vitro, are amenable to viral manipulation, and preferentially home to regions of ischemia found in growing tumors. We show here that human peripheral blood CEPs expanded ex vivo migrate to prostate cancer cells in vitro and efficiently home to human prostate tumor xenografts in vivo. Infection of precursors ex vivo with an adenovirus constructed to secrete a soluble form of the colony-stimulating factor-1 receptor CD115 that inhibits macrophage viability and migration in vitro significantly decreases the number of TAMs in xenografts (p < .05), reduces proliferation (p < .01) and vascular density (p < .03), and suppresses the growth of xenografts (p < .03). These data show for the first time that targeting stromal cell processes with cellular therapy has the potential to retard prostate tumor growth.
Authors:
Trevor Lucas; Dietmar Abraham; Gerold Untergasser; Karin Zins; Erhard Hofer; Eberhard Gunsilius; Seyedhossein Aharinejad
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Stem cells (Dayton, Ohio)     Volume:  27     ISSN:  1549-4918     ISO Abbreviation:  Stem Cells     Publication Date:  2009 Sep 
Date Detail:
Created Date:  2009-09-16     Completed Date:  2009-12-11     Revised Date:  2013-06-02    
Medline Journal Info:
Nlm Unique ID:  9304532     Medline TA:  Stem Cells     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2342-52     Citation Subset:  IM    
Affiliation:
Laboratory for Cardiovascular Research, Department of Anatomy and Cell Biology, Vienna Medical University, Vienna, Austria.
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MeSH Terms
Descriptor/Qualifier:
Adenoviridae / genetics*
Animals
Blotting, Western
Cell Line
Cell Line, Tumor
Cell Movement / genetics,  physiology
Cell Proliferation
Endothelial Cells / cytology,  metabolism*
Flow Cytometry
Humans
Male
Mice
Mice, Nude
Prostatic Neoplasms / metabolism,  therapy*
Receptor, Macrophage Colony-Stimulating Factor / genetics,  metabolism*
Stem Cell Transplantation / methods
Tissue Therapy / methods
Xenograft Model Antitumor Assays
Grant Support
ID/Acronym/Agency:
S 9403//Austrian Science Fund FWF
Chemical
Reg. No./Substance:
EC 2.7.10.1/Receptor, Macrophage Colony-Stimulating Factor
Comments/Corrections

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