| Adenosinergic modulation of neuronal activity in the pond snail Lymnaea stagnalis. | |
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MedLine Citation:
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PMID: 20228349 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Adenosine has been termed a retaliatory metabolite and its neuroprotective effects have been implicated in the hypoxia tolerance of several species; however, its role in the invertebrate CNS remains unclear. To determine if adenosine modulates neuronal activity in invertebrate neurons, we conducted whole-cell recordings from neurons in the central ring ganglia of the anoxia-tolerant pond snail Lymnaea stagnalis during exposure to adenosine and pharmacological compounds known to modulate the type I subclass of adenosine receptors (A(1)R). Action potential (AP) frequency and membrane potential (V(m)) were unchanged under control conditions, and addition of adenosine decreased AP frequency by 47% (from 1.08+/-0.22 to 0.57+/-0.14 Hz) and caused significant hyperpolarization of V(m). The A(1)R agonist cyclopentyladenosine (CPA) mimicked the results obtained with adenosine whereas antagonism of the A(1)R with 8-cyclopentyl-1,3-dipropylxanthine (DPCPX) had no effect on AP frequency or V(m) but prevented the adenosine and CPA-mediated decreases in neuronal activity. Furthermore, Ca(2+) measurements with fluo-4 revealed that A(1)R activation led to a 12% increase in intracellular Ca(2+) concentration and this elevation was also antagonized by DPCPX. Our results suggest that adenosine acting via the adenosine receptor (type I subclass) depresses neuronal activity in the adult L. stagnalis CNS and this depression is correlated with an increase in cytosolic Ca(2+) levels. |
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Authors:
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Aqsa Malik; Leslie Thomas Buck |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of experimental biology Volume: 213 ISSN: 1477-9145 ISO Abbreviation: J. Exp. Biol. Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-03-15 Completed Date: 2010-07-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0243705 Medline TA: J Exp Biol Country: England |
Other Details:
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Languages: eng Pagination: 1126-32 Citation Subset: IM |
Affiliation:
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Department of Cell and Systems Biology, University of Toronto, ON, Canada. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Action Potentials
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drug effects Adenosine / pharmacology* Animals Fresh Water* Lymnaea / drug effects*, physiology* Neurons / drug effects*, physiology* Receptors, Purinergic P1 / antagonists & inhibitors, metabolism* Seasons Xanthines / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Receptors, Purinergic P1; 0/Xanthines; 102146-07-6/1,3-dipropyl-8-cyclopentylxanthine; 58-61-7/Adenosine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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